摘要
利用中国农田开放式O3浓度升高(O3-FACE)平台,于2010年和2011年对O3浓度升高和田间自然条件下转Bt基因水稻Bt汕优63(Bt-SY63)及其常规水稻汕优63(SY63)根际土壤微生物群落功能多样性进行了研究.结果表明,O3浓度升高使Bt-SY63和SY63水稻土壤微生物总体活性有降低的趋势,O3浓度升高未使两基因型水稻土壤微生物的丰富度、优势度和均一度发生显著变化.O3浓度升高显著地改变了Bt-SY63土壤微生物碳源基质的利用方式,使其土壤微生物由对糖类及其衍生物具有较强的利用能力转向对代谢中产物和次生代谢物具有较强的利用能力,而对SY63土壤微生物碳源基质的利用没有显著影响.因此,从稻田土壤微生物群落对O3浓度升高的不同响应来看,Bt-SY63可能比SY63更敏感.
Using a free-air concentration enrichment(FACE) system, field studies were conducted on functional diversity of soil microbial community from two rice lines, transgenic Bt Shanyou 63(Bt-SY63) and its non-transgenic counterpart(SY63), in elevated O3(E-O3) versus ambient O3(A-O3) condition during 2010~2011. The results indicated that the AWCD(average well colour development) values of Bt-SY63 and SY63 tended to decrease as O3 increased. No significant ozone effects(P 0.05) were detected on Simpson, Shannon and Mc Intosh indices of functional diversity of the soil microbial communities in Bt-SY63 and SY63. Edaphon's ability to utilize the substrates of some specific carbon resources was significantly affected(P 0.05) by E-O3 in Bt-SY63 from the original carbohydrates and their derivants to the metabolic mediates and secondary metabolites, whereas SY63 was not significantly affected(P 0.05) by E-O3. The results suggested that Bt-SY63 might be more sensitive to E-O3 than SY63 with respect to the different responses to E-O3 in the soil microbial community.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2014年第11期2922-2930,共9页
China Environmental Science
基金
转基因新品种培育重大科技专项(2014ZX08012-005)
国家自然科学基金(31370544)
南京林业大学优秀博士学位论文创新基金项目(2011YB009)
江苏省普通高校研究生科研创新计划资助项目(CXZZ11_0504)
中国科学院知识创新方向项目(KZCX2-EW-414)