摘要
目的:应用同位素标记相对和绝对定量(i TRAQ)技术结合LC-MALDI-TOF/TOF MS方法筛选潜在的房间隔缺损血清标志蛋白,探索房间隔缺损的发病机制。方法:收集房间隔缺损患者和与之相匹配的室间隔缺损患者、健康人血清各20例,等量混合后使用多重亲和去除系统(MARS)去除14种高丰度蛋白,经i TRAQ试剂标记后应用二维液相色谱分离,然后利用质谱进行相对定量分析和生物信息学分析。结果:质谱鉴定出置信度>95%的蛋白质共256种,其中表达量差异有统计学意义(P<0.05)的蛋白质有22种,房间隔缺损组较室间隔缺损组和健康对照组表达量均上调>1.3倍的蛋白有8种,下调<0.77倍的蛋白有14种。结论:应用i TRAQ技术筛选出22种房间隔缺损相关差异性蛋白,这些蛋白可作为房间隔缺损血清蛋白标志物的重要候选因子。
Objective： To screen potential serum differentially expressed proteins of atrial septal defect （ASD） by isobaric tags for relative and absolute quantitation （iTRAQ） combined with LC - MALDI - TOF/TOF MS technology, explore the pathogenesis of ASD. Methods： The serum samples were respectively collected from 20 children with ASD and 20 healthy children, multiple affinity removal sys- tem （MARS） was used to delete 14 kinds of high abundant proteins, two- dimensional liquid chromatography was used after iTRAQ labeling, relative quantitative analysis and bioinformatics analysis were performed based on mass spectrum. Results： A total of 256 proteins with the confidence coefficient 〉 95% were screened, 22 kinds of proteins were differentially expressed proteins （P 〈 0. 05 ）, 8 proteins were up - regulated by 1.3 times and 14 proteins were down - regulated in ASD group compared with ventricalar septal defect group and healthycontrol group. Conclusion： A total of 22 differentially expressed proteins related to ASD were screened out by iTRAQ technology, which can be used as important candidate serum biomarkers of ASD.
出处
《中国妇幼保健》
CAS
北大核心
2014年第33期5498-5501,共4页
Maternal and Child Health Care of China
基金
广西自然科学基金重点项目〔2010GXNSFD013054〕
