摘要
目的 探讨梗阻性黄疸兔肝细胞线粒体氧化损伤的作用机制.方法 选择新西兰白兔30只,按照随机分配的原则分为梗阻性黄疸组24例和对照组6例,造模7d后,分别取各组家兔的肝组织各100 mg,对肝组织内的8-羟基-2-脱氧鸟苷酸（8-OHdG）和丙二醛（MDA）分别进行免疫组织荧光显像、酶联免疫吸附试验（ELISA）法和MDA含量检测.结果 （1）肝组织免疫组织荧光结果：梗阻性黄疸组呈现橘红色,对照组呈现绿色;（2）肝细胞线粒体内8-OHdG的含量：梗阻性黄疸组肝细胞内的8-OHdG的含量（1920 ±231） pg/mg蛋白明显高于对照组（210 ±78） pg/mg蛋白,差异有统计学意义（P＜0.01）;（3）肝细胞内MDA的含量：梗阻性黄疸组和对照组肝细胞内的MDA分别为（1.08±0.25）和（0.21±0.09） nmol/mg蛋白,前组明显高于后者,差异有统计学意义（P＜0.01）.结论 兔梗阻性黄疸可导致肝细胞线粒体内氧化应激损伤,其损伤机制可能与肝细胞线粒体内8-OHdG和MDA有关.
Objective To explore the mechanism of rabbit liver mitochondrial oxidative damage about obstructive jaundice.Methods The 30 New Zealand rabbits were divided into two groups,which were the control group and the obstructive jaundice group.After the rabbits were modeled for 7 days,they were taken in liver tissue 100 mg,which were detected the index——8-hydroxydeoxyguanosine （8-OHdG） and malondialdehyde （MDA）.Results （1） The results of fluorescence immunohistochemistry：the liver cells of rabbits in the obstructive jaundice group （pre-treatment） were found to have significant mitochondrial deoxyribonucleicacid （mtDNA） oxidative damage.（2） Levels of 8-OHdG in the liver mitochondria：the 8-OHdG levels of the control group and the obstructive jaundice group were （210 ± 78） pg/mg protein and （1920 ±231） pg/mg protein,There is significant difference between them （P 〈0.01）.（3） Levels of MDA in the liver mitochondria：the MDA levels of the control group and the obstructive jaundice group were （0.21 ± 0.09） nmol/mg protein and （1.08 ± 0.25） nmol/mg protein,There is significant difference between them （P 〈 0.01）.Conclusion The obstructive jaundice of rabbits can cause hepatic mitochondrial oxidative stress injury,which mechanism maybe remove 8-OHdG and MDA in the liver mitochondria.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第11期2393-2395,共3页
Chinese Journal of Experimental Surgery
基金
广东省自然科学基金资助项目（S2013010016323）
