摘要
目的:探讨预针刺和即刻针刺对模拟失重大鼠肾功能及肾脏氧自由基代谢的影响。方法:将20只雄性清洁级Wister大鼠随机分为正常对照组、模型组、预针刺组和即刻针刺组,每组5只。正常对照组不做任何处理,自由活动4周;模型组、预针刺组和即刻针刺组尾部悬吊4周制造模拟失重大鼠模型。预针刺组尾部悬吊前一周,电针刺激双侧"肾俞""脾俞"和"三阴交"穴,每次30min,每日1次,针刺7d;即刻针刺组针刺与尾部悬吊同时进行,大鼠尾部悬吊过程中,电针刺激"肾俞""脾俞"和"三阴交"穴,每次30min,隔日治疗1次,针刺14d。采用比色法检测各组大鼠干预后血清中尿素氮(BUN)含量和肾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)含量。结果:与正常对照组比较,模型组大鼠血清BUN的含量显著升高(P<0.01),肾组织中SOD和GSH-PX活性显著降低(均P<0.01),MDA含量显著增高(P<0.05);与模型组相比,预针刺组、即刻针刺组BUN含量均显著降低(P<0.01,P<0.05),预针刺组GSH-PX活性显著升高(P<0.05),即刻针刺组MDA含量显著升高(P<0.01);与即刻针刺组比较,预针刺组MDA含量较低(P<0.01)。结论:预针刺和即刻针刺都具有改善模拟失重大鼠肾功能和抗氧自由基损伤的功能,但预针刺组提高肾脏防御和清除自由基的能力优于即刻针刺组,其作用机制可能与提高肾脏抗氧化能力有关。
Objective To explore the effects of pre-acupuncture and immediate acupuncture on kidney function and oxygen free radical metabolism in rats with simulated weightlessness.Methods Twenty male clean-grade wister rats were randomly divided into a normal control group,a model group,apre-acupuncture group and an immediate acupuncture group,5rats in each one.The rats in the normal control group did not receive any treatment but free activities for 4weeks.The rats in the rest groups received 4-week tail suspension to establish the model of simulated weightlessness.One week before the tail suspension,the rats in the pre-acupuncture group were treated with electroacupuncture at＂Shenshu＂（BL 23）,＂Pishu＂（BL 20）and＂Sanyinjiao＂（SP 6）for 30 min per treatment,once a day for 7days.The rats in the immediate acupuncture group received tail suspension and acupuncture at the same time;during the tail suspension,the electroacupuncture was applied at＂Shenshu＂（BL 23）,＂Pishu＂（BL 20）and＂Sanyinjiao＂（SP 6）for 30 min per treatment,once every other day for 14 days.The colorimetric method was used to measure the content of blood urea nitrogen（BUN）in serum as well as activity of superoxide dismutase（SOD）and glutathione peroxidase（GSH-PX）and content of malonaldehyde（MDA）in renal tissue in each group.Results Compared with the normal control group,the content of BUN in the model group was increased significantly（P〈0.01）,the activity of SOD and GSH-PX in nephridial tissue was significantly reduced（both P〈0.01）,and the content of MDA was increased significantly（P〈0.05）.Compared with the model group,the content of BUN in the pre-acupuncture group and immediate acupuncture group was significantly reduced（P〈0.01,P〈0.05）,the activity of GSH-PX in the pre-acupuncture group was obviously increased（P〈0.05）and the content of MDA in the immediate acupuncture group was increased significantly（P〈0.01）.Compared with the immediate acupuncture group,the content of MDA in the pre-acupuncture group was lower（P〈0.01）.Conclusion The pre-acupuncture and immediate acupuncture both have the capacity to improve the kidney function and anti-oxygen free radical injury in rats with simulated weightlessness,however,the capacity to increase the protection ability of the kidney and eliminate free radical in the pre-acupuncture group is superior to that in the immediate acupuncture group,which is likely to be related with improving antioxidant ability of kidney.
出处
《中国针灸》
CAS
CSCD
北大核心
2014年第11期1106-1110,共5页
Chinese Acupuncture & Moxibustion
基金
航天医学基础与应用国家重点实验室研究基金资助项目:SMFA11B01
关键词
模拟失重
预针刺
即刻针刺
肾功能
氧化应激
simulated weightlessness
pre-acupuncture
immediate acupuncture
kidney function
oxidative stress
