摘要
目的：揭示产地、品种、采收部位和潮次、破壁技术对赤芝孢子粉和子实体中总三萜、总多糖、总蛋白含量的影响。方法：收集龙芝1号、沪农1号、市售赤芝孢子粉和子实体,分别采用硫酸-蒽酮比色法、香草醛-冰醋酸比色法和Lowry法测定其中总多糖、总三萜、总蛋白的含量。结果：全国6个主产区的破壁孢子粉总多糖为0.40%~2.25%,总三萜为1.36%~3.15%,总蛋白为0.74%~1.91%;浙江主栽赤芝品种子实体总多糖、总三萜、总蛋白质量分数分别为0.25%~1.42%,0.44%~1.42%,1.82%~3.67%,未破壁孢子粉分别为0.41%~0.91%,0.09%~0.12%,0.78%~0.90%,破壁孢子粉分别为1.03~2.25%,1.89%~3.15%,0.96%~1.04%。结论：全国不同主产区的灵芝破壁孢子粉化学成分含量存在显著差异,浙江产区总多糖和总三萜含量较高,福建产区总蛋白含量较高;浙江主栽赤芝品种龙芝1号总三萜含量较高,沪农1号总多糖含量较高;破壁孢子粉中总三萜与总多糖含量高于子实体,子实体菌柄中总多糖含量大多高于菌盖,菌盖中总三萜含量显著高于菌柄;2潮采收的菌盖和菌柄中总多糖含量高于1潮相应的部位,但总三萜含量大多低于1潮;孢子粉破壁可显著提高总三萜和总多糖的提取和溶出率。
Objective： To reveal the quality variation of polysaccharides, triterpenoids and proteins in spores and fruiting bodies of Ganoderma lucidum from producing areas, different varieties, harvesting parts and periods, and wall-breaking treatments. Method： Spores and fruiting bodies from varieties of Longzhi No. 1 and Hunong No. 1 were collected as test samples, together with wall-broken spores sold in domestic main producing areas. The anthrone-suffuric acid colorimetric method was used to determine the content of total polysaccharides. The vanillin-glacial acetic acid-perchlorie acid colorimetric method was used to determine the content of total triterpenoids. The Lowry method was used to determine the content of total proteins. Result： The content ranges of total polysaccharides, total triterpenoids, and total proteins from 6 domestic main producing areas were 0. 40% -2. 25%, 1.36% -3.15% and 0. 74% -1.91% respectively. The content ranges of total polysaccharides, triterpenoids, and proteins in the fruiting bodies from 2 varieties cultured in Zhejiang were 0. 25% -1.42%, 0.44% -1.42% and 1.82% -3.67% respectively. In addition, the ranges of samples from wall-unbroken spores were 0. 41% -0. 91% , 0. 09% -O. 12% , O. 78% -0. 90% respectively and wall-broken spores are 1.03% - 2. 25% , 1.89% -3.15% , 0.96% -1.04% respectively. Conclusion： There are significant differences in the contents of main chemical ingredients of wall-broken G. lucidum spores saled in the markets. The samples from Zhejiang contain high content of total polysaccharides and triterpenoids, and samples from Fujian contains more proteins. Between the 2 major varieties cultured in Zhejiang, Longzhi No. 1 contains higher content of triterpenoids, but Hunong No. 1 has more polysaccharides. Contents of triterpenoids and polysacchatides from wall-broken spores are much higher than those of fruiting bodies. The stipes from fruiting bodies contains more polysaccharides than those of the pileus, while the triterpenoids contents are higher in the pileus than stipes. The pileus and stipes collected in the second year contain higher content of polysaccharides than the first year＇s samples, but the contents of triterpenoids are lower. Wall- breaking treatment would significantly improve the extraction and dissolution rate of total triterpenoids and polysaeeharides.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第21期4246-4251,共6页
China Journal of Chinese Materia Medica
基金
浙江省重大科技项目(2009C02005)
关键词
灵芝
孢子粉
子实体
含量测定
Ganoderma lucidum
spore
fruiting body
determination
