摘要
目的:分析艳山姜挥发油(EOFAZ)保护脂多糖(LPS)诱导人脐静脉内皮细胞(HUVECs)损伤的主要活性组分。方法:将EOFAZ中含量较高的4个成分(α-蒎烯,β-蒎烯,1,8-桉叶油醇,莰烯)作为考察因素,每个因素选取有或无共2个水平,按L8(27)正交表设计分组与给药。运用四甲基偶氮唑盐(MTT)法和NO检测法,以细胞活力和细胞分泌NO为指标分析EOFAZ的主要药效物质基础。采用单成分分析法测定各成分不同浓度给药保护后细胞活力及培养上清液NO含量,观察4个成分对LPS诱导损伤的HUVECs的保护作用。结果:挥发油中4个成分均能明显提高细胞存活率,保护作用主次顺序为莰烯>α-蒎烯>1,8-桉叶油醇>β-蒎烯,4个成分间均存在明显差异。单成分分析表明4个成分作用于HUVECs后,可提高细胞活力及培养上清液NO含量,且呈一定剂量相关性。结论:EOFAZ对LPS诱导损伤的HUVECs具有保护作用,其中莰烯,α-蒎烯,1,8-桉叶油醇和β-蒎烯为主要药效组分。
Objective:To analyze main ingredients of essential oil from Fructus Alpiniae Zerumbet(EOFAZ)against human umbilical vein endothelial cells(HUVECs)injury induced by lipopolysaccharide(LPS).Method:Four factors were selected from EOFAZ(α-pinene,β-pinene,1,8-eucalyptol,camphene)as research object,each factor was selected two levels,including contained or not contained,experiment was arranged according to Ls(27)Orthogonal test table.MTT activities and NO detection were used as indexes and data were analyzed by SPSS software.In component analysis method,protective effects of ingredients with different concentrations were assayed by testing cell viability and NO contents in medium.Result:Protective extent of four factor was camphene>α-pinene>1,8-eucalyptol>β-pinene,there were significant differences among these ingredients.Single component analysis showed that pretreatment with ingredients at different concentrations could obviously enhance cell viabilites and NO contents,and effects had dose-dependent.Conclusion:EOFAZ has a protective effect on HUVECs injury induced by LPS,and camphene,α-pinene,1,8-eucalyptol,β-pinene are main active components.
作者
张彦燕
令狐克刚
陈妍
陶玲
张小金
文波
沈祥春
ZHANG Yan-yan;LING-HU Ke-gang;CHEN Yan;TAO Ling;ZHANG Xiao-jin;WEN Bo;SHEN Xiang-chun(Key Laboratory of Optimal Utilization of Natural Medicinal Resources,Guiyang Medical University,Guiyang 550004,China)
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第20期9-12,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81360650,81102664)
贵州省科学技术基金项目(黔科合J字[2011]2285号)
贵州省卫生厅科学技术基金项目(gzwkj2011-1-019)
贵州省中药现代化科技产业研究开发专项(黔科合中药字[2012]5051号)
贵州省大学生创新创业训练项目(021203102)
关键词
艳山姜挥发油
脂多糖
人脐静脉内皮细胞
单成分分析法
细胞活力
essential oil from Fructus Alpiniae Zerumbet
lipopolysaccharide
human umbilical vein endothelial cells
single component analysis
cell viability
