摘要
为了解鸭源沙门菌江苏分离株的主要生物学特性,本研究对2012年至2013年从发病或死亡鸭中分离的33株沙门菌分离株进行鉴定,即采用玻片凝集法对分离的沙门菌进行血清学分型,多重PCR方法检测17种毒力基因的分布,按照美国临床和实验室标准化研究所制定的方法进行药物敏感性检测,通过结晶紫半定量法检测分离菌株的生物被膜形成能力。33株鸭源沙门菌的血清学分型结果显示,查理沙门菌占48.5%,为优势血清型。毒力基因检测结果显示,pagC、msgA、sipB、prgH、spaN、tolC、iroN、sopB及pefA为保守基因。药物敏感性检测显示,42.4%的菌株耐受8种以上药物。生物被膜检测显示,有14株细菌生物被膜形成能力为中等以上,其中57.1%的菌株耐8种以上的药物。
To investigate the biological characteristics of prevalent Salmonella from ducks in Jiangsu province, a total of 30 isolates of Salmonella were isolated from affected ducks in different farms during 2012 to 2013, which were subjected to serological typing by slide agglutination test, virulence genes detecting by multiplex PCR, drug susceptibility testing according to Clinical and Laboratory Standards Institute （CLSI）, and biofilm forming ability estimation through improved crystal violet semi-quantitative method. The results showed that S.chailey was the main serotype （16/33）, and the pagC, msgA, sipB, prgH, spaN, tolC, iroN, sopB and pefA were the conserved virulence gene profiling among the isolates. In addition, 42.4% （14/33） of the isolates were resistant to more than 8 antibiotics. Moreover, fourteen isolates produced moderate or strong biofilm in vitro, of which 57.1% were resistant to more than 8 antibiotics.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2014年第10期775-779,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
公益性行业(农业)科研专项(201303044)
农业部948项目(2013-Z56)
国家自然科学基金(31402200)
江苏省自然科学基金(BK20141282)
江苏省农业科技自主创新项目[CX(12)3075]
关键词
沙门菌
鸭
血清型
毒力基因
耐药性
生物被膜
Salmonella
duck
serotypes
virulence genes
drug resistance
biofilm formation
