摘要
Gut microbiota play important roles in host health and diseases.Several factors,in particular antibiotics,affect the gut microbiota of pigs.Cross-fostering has been applied as a regular practice to equalize litter size,reduce pre-weaning mortality and increase body weight.However,the effect of cross-fostering on cecal microbiota is unclear.In this study,we fostered three Jinhua pigs to a Yorkshire sow.The fostered Jinhua piglets grew significantly faster than their biological siblings.To explore whether the cecal microbiota of piglets will alter during fostering,we characterized the cecal microbiota of allpiglets by examining the V3 hypervariable region of 16S rDNA.We observed altered cecal microbiota in these piglets using the Illumina HiSeq 2000 platform,and this was accompanied with an increase in growth rate after fostering.The relative abundance of Bacteroidetes in the fostered Jinhua piglets was decreased compared with their biological siblings,although still higher in comparison with their new littermates.Beta-diversity analysis also showed that the cecal microbiota of the adopted Jinhua piglets differed from their biological siblings with a shift toward their step-siblings.Our data show that cecal microbiota of piglets were altered after cross-fostering while the growth rate increased.
Gut microbiota health and diseases. Several play important roles in host factors, in particular antibiot- ics, affect the gut microbiota of pigs. Cross-fostering has been applied as a regular practice to equalize litter size, reduce pre-weaning mortality and increase body weight. However, the effect of cross-fostering on cecal microbiota is unclear. In this study, we fostered three Jinhua pigs to a Yorkshire sow. The fostered Jinhua piglets grew signifi- cantly faster than their biological siblings. To explore whether the cecal microbiota of piglets will alter during fostering, we characterized the cecal microbiota of allpiglets by examining the V3 hypervariable region ot 165 rDNA. We observed altered cecal microbiota in these piglets using the Illumina HiSeq 2000 platform, and this was accompanied with an increase in growth rate after fostering. The relative abundance of Bacteroidetes in the fostered Jinhua piglets was decreased compared with their biological siblings, although still higher in comparison with their new littermates. Beta-diversity analysis also showed that the cecal microbiota of the adopted Jinhua piglets differed from their biological siblings with a shift toward their step-siblings. Our data show that cecal microbiota of piglets were altered after cross-fostering while the growth rate increased.
基金
supported by the National Special Foundation for Transgenic Species of China(2014ZX0800950B,2011ZX08006-003)
the Specialized Research Fund of Ministry of Agriculture of China(NYCYTX-009)
the Fundof Fok Ying-Tung Education Foundation(141117)
the Fund for Distinguished Young Scientists of Sichuan Province(2013JQ0013)
the Program for Changjiang Scholars and Innovative Research Teamin University(IRT13083)to Mingzhou Li and Xuewei Li
the‘‘100-Talent Program’’in Sichuan Science Foundation for Youths of Sichuan Province to Ying Li
