摘要
目的:研究miR-93对卵巢癌细胞系OVCAR3侵袭及迁移能力的影响。方法:用miR-93的成熟模拟物过表达miR-93,用化学合成的特异性抑制物下调miR-93的表达。通过miRNA特异的定量PCR验证miR-93的过表达和抑制效率,采用MTT、软琼脂集落形成和Transwell侵袭实验分析miR-93表达对OVCAR3细胞增殖、侵袭及迁移能力的影响。结果:miR-93过表达后,OVCAR3细胞的侵袭和迁移能力显著增强(分别达3.82倍和2.56倍)。降低内源性miR-93的表达后,OVCAR3细胞的侵袭和迁移能力分别下降了66.57%和57.26%。结论:miR-93能明显促进卵巢癌OVCAR3细胞的侵袭和迁移。
Objective：To verify the effect of miR-93 on the invasive and migratory abil- ity of human ovarian cancer cell line OVCAR3. Methods： The expression of miR-93 in OVCAR3 cells was upregulated by mimics and downregulated by inhibitors. The expression levels of miR-93 were measured by miRNA specific Real-Time PCR. Cell proliferation was detec- ted with MTT assay and sofl-agar colony formation assay. Effects of miR-93 on cell invasion and migration were evaluated by transwell invasive and migratory assay. Results：Overexpression of miR-93 in OVCAR3 cells, the invasive and migratory abilities were increased by 3.82-fold and 2.56-fold respectively. On the contrary,inhibition of endogenous miR-93 in OVCAR3 cells, the migration and invasion of cells were reduced by 57.26% and 66.57% respectively in vitro. Conclusion：miR-93 plays an important role in promoting invasion and migration of human o- varian cancer cell line OVCAR3.
出处
《现代妇产科进展》
CSCD
2014年第9期681-684,共4页
Progress in Obstetrics and Gynecology
