摘要
分析陆地棉栽培种遗传多样性,通过关联分析寻找与棉花农艺性状相关联的分子标记,为分子标记辅助选择育种和提高棉花育种效率奠定基础。本文采用74个Simple sequence repeat（SSR）标记对172份陆地棉栽培种的基因组变异进行扫描,使用NTSYS-pc 2.20进行聚类,分析该群体遗传多样性;利用Structure 2.3.4软件分析群体结构,在此基础上结合田间表型数据,采用Tassel 2.1的一般线性模型（General linear model,GLM）进行关联分析,定位与农艺性状相关的QTLs。74个标记共检测到148个多态性位点,涉及246个等位变异,变异范围2~7个,平均等位变异数为3.32;引物的多态性信息含量（PIC）为0.0281-0.3733,平均值为0.2370;遗传相似系数变异在0.2816-1,平均值为0.5369,平均遗传相似系数为0.5369,表明我国陆地棉遗传基础狭窄,尽管国外及西北内陆棉区部分材料具有较丰富的遗传变异。聚类分析将该群体划分为12个亚群,不同棉区的材料交叉分布,且聚类结果基本与系谱吻合。群体结构分析却将172份供试材料划分为3个亚群;通过关联分析,发现30个位点与铃重、衣分、黄萎病抗性显著相关（P〈0.05）,各位点对表型变异贡献率为2.24%-5.27%。
To provide a genetic basis for molecular-assisted breeding and to improve the efficiency of breeding, simple sequence repeat（SSR） markers associated with agronomic traits were screened and the genetic diversity of cultivated upland cotton was analyzed. In 172 upland cotton cultivars, 74 SSR markers were assayed for polymorphism, and cluster analysis was performed on the genetic similarity matrix using NTSYS-pc 2.20 software to ascertain the diversity. Based on the analysis of population structure, an association analysis between SSR markers and three agronomic traits were performed using the Tassel 2.1 GLM （general linear model） program. The 74 SSR markers included 246 alleles belong to 148 loci, with an average of 3.32 alleles per SSR marker（range, 2-7）. The average polymorphic information content（PIC） for per marker was 0.2730（range, 0.0281-0.3733）, and the mean genetic similarity was 0.5369（range, 0.2816-1 ）. This revealed low levels of upland cotton genetic diversity in China, although some oversea varieties and some varieties of the northwest inland cotton showed abundant genetic variation. Cluster analysis showed that all the accessions could be divided into 12 subgroups, which was in agreement with the pedigree but not with the origin. Structure analysis indicated that the 172 lines were assigned to three substructures. Of the SSR loci, 30 were significantly associated with boll weight, lint percentage, and Verticilium wilt resistance under GLM program, and the ratio of explanation on the phenotype variation of related markers ranged from 2.24% to 5.27%.
出处
《棉花学报》
CSCD
北大核心
2014年第5期420-430,共11页
Cotton Science
基金
国家科技支撑计划(2011BAD35B05)
中央级公益性科研院所基本科研业务专项(1610162014009)
关键词
陆地棉
关联分析
SSR
遗传多样性
群体结构
upland cotton
associate analysis
SSR
genetic diversity
population structure
