摘要
用荧光光谱法研究了模拟生理环境pH=7.40的条件下槲皮素与牛血清白蛋白和β-环糊精的相互作用.利用Stern-Volmer方程和Lineweaver-Burk方程对槲皮素猝灭牛血清白蛋白的荧光强度变化进行拟合,确定其荧光猝灭机理.利用修正的Benesi-Hildebrand方程对槲皮素的荧光强度随β-环糊精浓度的变化进行拟合,确定包合物的稳定常数和包合比.研究结果表明:槲皮素对牛血清白蛋白的荧光猝灭为静态猝灭过程.310K时,槲皮素与β-环糊精形成的包合物的稳定常数为66.7L·mol-1,包合比为1∶1.
The interaction of quercetin with bovine serum albumin andβ-cyclodextrin was studied by the methods of fluorescence spectroscopy under simulating physiological environments of pH=7.40.The quenching mechanism of fluorescence of BSA was obtained by fitting fluorescence intensity change according to the Stern-Volmer equation and the Lineweaver-Burk equation.The stability constant and the inclusion ratio of inclusion complex were obtained by fitting fluorescence intensity change of quercetin according to the modified Benesi-Hildebrand equation.The results show that the quenching mechanism of fluorescence is static quenching process.The stability constant of quercetin andβ-cyclodextrin complex is 66.7L·mol-1,and the inclusion ratio is 1∶1.
出处
《湖州师范学院学报》
2014年第8期30-34,共5页
Journal of Huzhou University
基金
湖州师范学院2013年校级课题(2013121)
浙江省自然基金项目(LQ12B03001)
关键词
荧光光谱法
槲皮素
牛血清白蛋白
Β-环糊精
相互作用
fluorescence spectroscopy
quercetin
bovine serum albumin
β-cyclodextrin
interaction
