摘要
以草菇菌丝体为试材,以凝集素提取液的总活力为指标,在单因素试验的基础上,利用3因素3水平的Box-Benhnken中心组合试验和响应面分析法设计优化了草菇菌丝凝集素的超声波提取工艺。结果表明:超声功率为310W、超声处理时间为36min、液料比为4.5∶1,是草菇菌丝凝集素超声波提取的最佳工艺;在该条件下,其20g草菇菌丝的凝集素提取液的总活力为76 386U。
Taking mycelium of Volvariella volvacea as material,with total lectin activity of extracting solution as the indicator,based on the single factor experiments,the ultrasonic-assisted extraction technology of Volvariella volvacea mycelium lectin was optimization by Box-Benhnken central composite design and response surface methodology.The results showed that the optimal extraction conditions were ultrasonic power 310 W,extraction time 36 min and liquid-solid ratio 4.5∶1.Under these conditions,the total lectin activity of extracting solution of 20 g Volvariella volvacea mycelium was 76 386 U.
出处
《北方园艺》
CAS
北大核心
2014年第19期138-141,共4页
Northern Horticulture
基金
韶关学院科研资助项目(2011-12)
韶关学院大学生创新创业训练计划资助项目(sycxcy2012-007)
关键词
草菇菌丝
凝集素
超声波提取
响应面分析
Volvariella volvacea mycelium
lectin
ultrasonic-assisted extraction
response surface analysis
