摘要
目的测定槲皮素、根皮苷、杨梅素、原花青素、葛根素和柚皮苷对超氧阴离子自由基(O-2·)的清除能力,探讨6种化合物清除O-2·的构效关系。方法采用流动注射邻苯三酚-鲁米诺化学发光法测定6种化合物对O-2·的清除率,采用IC50来衡量样品对自由基的清除能力。以维生素C为阳性对照。结果槲皮素、根皮苷、杨梅素、柚皮苷、葛根素、原花青素对O-2·的抑制率分别为0.1547、0.2048、0.4623、0.6157、1.8098、2.9798μmol/L。6种化合物清除O-2·能力从大到小依次为槲皮素、根皮苷、杨梅素、柚皮苷、葛根素、原花青素,均高于维生素C(4.9586μmol/L)。结论 6种黄酮类化合物结构与清除O-2·能力密切相关:酚羟基是其清除O-2·的必要基团,B环的羟基化程度、羟基的数目和位置,C环开环后形成的羟基和双键,A环取代基的位置、数目以及化合物的空间结构,均是影响抗氧化活性的重要因素。
Objective To study the scavenging superoxide anion free radical （O-2·） ability of quercetin, myricetin, phlo- ridzin, naringin, puerarin and proanthocyanidins, and to explore their structure-activity relationships for removing 0-2·. Methods The scavenging 0-2· ability was determined by flow injection analysis-pyrogallol-chemiluminescence. IC50 was calculated and used to examine the scavenging free radical ability. Vitamin C was used as positive＇ control. Results The results showed that IC50 of quercetin, myricetin, phloridzin, naringin, puerarin and proanthocyanidins were 0.1547, 0.2048, 0.4623, 0.6157, 1.8098, 2.9798 μmol/L, respectively. The scavenging O-2· ability of the 6 compounds were ar- ranged as quercetin, myricetin, phloridzin, naringin, puerarin and proanthocyanidins, and all were higher than Vitamin C （4.9586 μmol/L）. Conclusion The scavenging 0-2·ability of 6 flavonoids compounds have been closely related to their structures： phenolic hydroxyl is the necessary group for removing the O-2·, the degree of hydroxylation, the number and location of hydroxyl of B ring, the hydroxyl and double bond of the formatting of C ring opening, substituent posi- tion, number and spatial structure of A ring, are all important influencing factors of antioxidant activity.
出处
《中国医药导报》
CAS
2014年第29期7-10,14,共5页
China Medical Herald
基金
山东省自然科学基金资助项目(编号ZR2011BL007)
泰山医学院高层次课题培育计划项目(编号2013GCC05)
关键词
6种黄酮类化合物
超氧阴离子自由基
清除率
构效关系
Six flavonoids compounds
Superoxide anion free radical
The scavenging ability
The Structure-activity relationships
