摘要
利用核酸适配体特异的分子识别功能以及特定荧光基团之间的能量转移,建立了一种高灵敏度、高选择性测定雌二醇的光谱方法。研究了缓冲溶液的pH值、组成和浓度、核酸浓度、实验温度及响应时间等因素对检测雌二醇的影响。在最优的实验条件下（50 mmol/L BR缓冲溶液（pH 7.4）,1.0×10^-7mol/L核酸,实验温度45℃,响应时间19 min）,体系荧光强度的改变值ΔI与雌二醇浓度的对数lgC呈良好的线性关系（r=0.9953）,线性范围为1.0×10^-11~5.0×10^-9mol/L,检出限达6.0×10^-12mol/L（S/N=3）。将本方法用于检测人体尿液中雌二醇的含量,雌二醇的加标回收率为94.0%~103.5%。
A highly sensitive fluorescence spectroscopic method was established for the selective determination of estradiol, which took advantages of the excellent molecular recognition capability of aptamer and the energy transfer between the specific fluorescent groups. The effects of the pH value, buffer constituent and concentration, the concentration of DNA, the experimental temperature and response time on the detection of estradiol were studied. Under the optimal conditions （50 mmol/L BR buffer solution with pH value at 7.4, 1.0×10^-7 mol/L for each DNA strand, incubation at 45℃, response time 19 min）, the change of the fluorescence intensity （AI） versus the logarithm of the concentration of estradiol （lgC） was linear over a concentration range from 1.0×10^-11 mol/L to 5.0×10^-9 mol/L with good linear correlation （r=0. 9953）. The limit of detection （LOD） was found to be 6.0×10^-12 mol/L （S/N= 3 ）. This method was successfully applied to the detection of estradiol in human urine, with the recovery in the range of 94.0%-103.5%. This method showed good precision and accuracy.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2014年第9期1295-1300,共6页
Chinese Journal of Analytical Chemistry
基金
广东省高等学校"千百十工程"人才培养专项基金(No.01080106)
深圳市战略性新兴产业发展专项资金项目(No.2113K3070038)资助
关键词
适配体
荧光共振能量转移
雌二醇
Aptamer
Fluorescence resonance energy transfer
Estradiol
