摘要
目的采用反转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测咬干扰对实验大鼠颞下颌关节软骨的炎性因子表达。方法选用6周龄雌性SD大鼠9只,随机分为咬紊乱组、去除咬紊乱组和正常对照组,每组3只。制造咬干扰动物模型。6周后,咬紊乱组不做处理,去除咬紊乱组去除咬干扰,正常对照组不做处理。8周后切取大鼠右侧颞下颌关节,采用RT-PCR方法检测颞下颌关节软骨中白细胞介素1(interleukin-1,IL-1),白细胞介素6(interleukin-6,IL-6),肿瘤坏死因子α(tumornecrosis factorα,TNF-α)及γ干扰素(interferon-γ,IFN-γ)的表达。结果在咬干扰条件下,RT-PCR检测显示炎性因子IL-1、IL-6及TNF-α在各组大鼠中均有表达,但咬紊乱组和去除咬紊乱组的炎性因子表达均高于对照组,咬紊乱组IL-6和TNF-α的表达高于去除咬紊乱组;3组大鼠均未检测到IFN-γ的表达。结论咬干扰条件下,炎性因子IL-1、IL-6及TNF-α在颞下颌关节软骨中的表达增强,提示炎性因子与实验大鼠髁突软骨病理性改建活动有相关性。
Objective Reverse transcription polymerase chain reaction（RT-PCR）was used for inflammatory factor expression in experimental rat temporomandibular joint cartilage by the occlusal interference. Methods The six week old female SD 9 rats were randomly divided into occlusal disorder group,removed occlusal disorder group and normal control group,3 rats in each group. The occlusal interference animal model was established. After six weeks,occlusal disorder group did not receive any treatment,removed occlusal disorder group was rid of occlusal interference,normal control group served as normal control. After eight weeks,the rats were sacrificed,the right side of the temporomandibular joint was cut out. RT-PCR was used to detect interleukin-1（IL-1）,interleukin-6（IL-6）,tumor necrosis factorα（TNF-α）,and interferon -γ（IFN-γ）expression in the temporomandibular joint cartilage. Results In occlusal interference condition,RT-PCR results showed inflammatory cytokines IL-1,IL-6 and TNF-α in rats of different groups were expressed. However,the inflammatory cytokines expression of the experimental group were higher than those of control group. IL-6 and TNF-α expression in occlusal disorder group were higher than those of removed occlusal disorder group. IFN-γ expression in three groups was not detected. Conclusion Under the occlusal interference condition,the expression of the inflammatory cytokines IL-1,IL-6 and TNF-α in the temporomandibular joint cartilage got enhancement. These results suggested that inflammatory cytokines and temporomandibular joint condylar remodeling in rats activity correlated.
出处
《河北医科大学学报》
CAS
2014年第8期897-900,共4页
Journal of Hebei Medical University
关键词
错[牙合]
颞下颌关节障碍
基因表达
malocclusion
temporomandibular joint disorders
gene expression
