摘要
目的观察标本溶血对生化检验结果的干扰和影响,根据研究结果制定相应的对策。方法选取体检者58例作为研究对象。空腹抽取静脉血5mL左右,经过自然凝固和离心后测定血液中的K+、Na+、总蛋白质(TP)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、天门冬氨酸氨基转移酶(AST)、清蛋白(ALB)、三酰甘油(TG)、高密度脂蛋白(HDL)、乳酸脱氢酶(LDH)以及α-羟基丁酸脱氢酶(HBDH)等生化指标。然后将标本血清溶液溶血后再测定以上各项生化指标,对比溶血前、后的测定结果。结果溶血前、后测定的各项生化指标中K+、Na+、TP、CK、CK-MB、AST、LDH以及HBDH等比较差异有统计学意义(P<0.05);溶血前、后测定的各项生化指标中ALB、HDL以及TG等比较差异无统计学意义(P>0.05)。经过回归分析,K+、Na+、TP、CK、CK-MB、AST、LDH以及HBDH等指标与溶血有关。结论标本溶血对生化检验结果中的K+、Na+、TP、CK、CK-MB、AST、LDH以及HBDH等造成影响,利用血清血红蛋白(Hb)浓度校对具有一定的应用价值。
Objective To observe the interference and influence of sample hemolysis on biochemical test results and to make cor-responding countermeasures based on the research results .Methods There were 58 cases of people who underwent physical exami-nation in the hospital .They were selected as study objects .Sample of venous blood was 5 mL in fasting .After natural coagulation and centrifugation ,K＋ ,Na＋ in blood were measured ,followed by the TP ,CK ,CK-MB ,AST ,ALB ,TG ,HDL ,LDH ,HBDH and other biochemical indexes .Then all indexes mentioned above were detected again after the sample hemolysis of serum ,and analysis results were compared between them .Results Of biochemical indicators detected before and after the determination of hemolysis , there were of statistical significance in the differences in K ＋ ,Na＋ ,TP ,CK ,CK-MB ,AST ,LDH and HBDH（P〈0 .05）;but there were no significant differences in biochemical indicators like ALB ,HDL and TG（P〉0 .05） .Through regression analysis ,biochemi-cal indicators such as K ＋ ,Na＋ ,TP ,CK ,CK-MB ,AST ,LDH and HBDH were found to be related to hemolysis .Conclusion Sample hemolysis has certain influences on the results of biochemical test in terms of K ＋ ,Na＋ ,TP ,CK ,CK-MB ,AST ,LDH and HBDH , which is of certain application value with the proofreading of serum Hb concentration .
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第22期2879-2880,2883,共3页
Chongqing medicine
关键词
标本溶血
生化检验
影响因素
研究对策
sample hemolysis
biochemical test
influencing factors
countermeasures
