摘要
目的分析和探讨乙型肝炎病毒(HBV)血清标志物ELISA法检测条件的优化途径。方法比较洗板机洗板后用蒸馏水或干净自来水冲洗反应板与不冲洗反应板的临界值标本比例。结果洗板后用蒸馏水再次冲洗反应板数秒后、用干净自来水再次冲洗反应板与不冲洗反应板的表面抗原临界值标本比例(含临界阴性和弱阳性)分别为0.52%、2.60%和5.56%;表面抗体临界值标本比例(含临界阴性和弱阳性)分别为5.52%、8.02%和14.86%;e抗原临界值标本比例(含临界阴性和弱阳性)分别为2.82%、2.92%和3.55%。结论洗板结束后用蒸馏水或干净自来水再次冲洗反应孔边缘可降低因酶标试剂或显色剂污染反应孔边缘导致的高临界值标本比例。蒸馏水比干净自来水更适合作为反应孔边缘的冲洗液。
Objective To analyze and approach the optimizing of ELISA assay for HBV serum markers.Methods To compare the critical value rate of flushing with distilled water,flushing with cleaning tap water and flushing without anything.Results The HBsAg critical value rates of flushing with distilled water,flushing with cleaning tap water and flushing without anything were 0.52%,2.60% and 5.56%.The HBsAb critical value rates of flushing with distilled water,flushing with cleaning tap water and flushing without anything were 5.52%,8.02% and 14.86%.The HBeAg critical value rate of flushing with distilled water,flush-ing with cleaning tap water and flushing without anything were 2.82%,2.92%and 3.55%.Conclusion Flushing with distilled wa-ter or flushing with cleaning tap water can reduce the high critical value rate because of enzyme or developer pollution.Flushing with distilled water exhibits more efficacy than flushing with cleaning tap water.
出处
《国际检验医学杂志》
CAS
2014年第12期1618-1619,共2页
International Journal of Laboratory Medicine
关键词
乙型肝炎病毒
酶联免疫吸附测定
临界值
hepatitis B virus
enzyme linked immunosorbent assay
critical value
