摘要
目的:探讨姜黄素对自发性高血压大鼠(SHR)脑缺血/再灌注后认知功能及海马神经元损伤和调解活化正常T细胞表达和分泌的趋化因子(RANTES)表达的影响。方法:雄性Wistar-Kyoto大鼠(WKY)和SHR,随机分为5组:假手术组(W-Sham、S-Sham)、缺血/再灌注组(W-I/R、S-I/R)和姜黄素组(S-Cur),各组按再灌注时间分为3h、12 h、1 d、3 d、7 d 5个亚组(n=6)。采用四血管阻断法制备全脑缺血/再灌注模型,HE染色观察海马CA1区神经细胞形态,Nissl染色计数海马CA1区平均锥体细胞密度,ELISA法检测海马RANTES表达,于再灌注后7 d观察行为学。结果:与假手术组大鼠比较,缺血/再灌注组大鼠学习和记忆能力下降,海马CA1区神经元损伤加重,海马RANTES蛋白表达上调(P<0.05);与W-I/R大鼠比较,S-I/R大鼠学习和记忆能力下降,海马CA1区神经元损伤加重,海马RANTES蛋白表达上调(P<0.05);姜黄素组大鼠学习和记忆能力明显改善,海马CA1区神经元损伤减轻,海马RANTES蛋白表达下调(P<0.05)。结论:缺血/再灌注更易导致SHR海马神经元损伤。姜黄素减轻SHR脑缺血/再灌注海马神经元损伤,其机制可能与抑制RANTES蛋白的表达有关。
Objective： To investigate the effect of curcumin on the injury in hippocampal neurons and the expression of regulated upon activation normal T-ceU expressed and secreted （RANTKS） in hippocamp during cerebral isehemia/repeffusion （I/R） in rots with spontaneous hypertension （SH）. Methods： Male Wistar-Kyoto （WKY） rats and spontaneous hypertension rats （SHR） were randomly divided into five groups （ n = 6）： sham group （W-Sham and S-Sham group）, ischemia/reperfusion group （W-I/R and S-I/R group）, curcumin group （S-Cur group）. Each group was splitted into 5 subgroups of 3 h, 12 h, 1 d, 3 d and 7 d according to the time interval before reperfusion. Global brain isehemia/reperfusion model was established by 4-VO method. Hematoxylin-eosin staining （HE staining） was used to observe the vertebral cell morphology in hippocampal CA1 region. Nissl staining was applied to detect the average density of cone cells in hippocampal CA1 region. The expression of RANTES in hippocamp was determined by ELISA. The behavior of the rats was evaluated at 7 days after reperfusion. Results： Compared with the sham group rats, the ability of learning and memory was significantly decreased in ischemia/reperfusion group rats, the number of injured neurons were greatly elevated , the protein expression levels of RANTKS was significantly increased（ P 〈 0.05）. Compared with W-I/R group rats, the ability of learning and memory in S-I/R group rats was greatly reduced, the number of injured neurons increased extremely, the protein expression level of RANTES was significantly enhanced（ P 〈 0.05）. The number of injured neurons declined significantly in S-Cur group rats, the ability to learn and remember of these rats was improved and the RAhrlYS protein content decreased significantly（ P 〈0.05）. Conclusion： SHR are more susceptible to ischemia/reperfusion induced hippocampal neuronal injury which may be improved by curcumin. Its underlying mechanism is possibly associated with the inhibition of RANTES protein expression level.
出处
《中国应用生理学杂志》
CAS
CSCD
2014年第4期360-364,367,I0002,共7页
Chinese Journal of Applied Physiology
基金
浙江省科技厅(2011C23022)
