摘要
目的:了解运动训练对原发性高血压大鼠骨骼肌血管调节因子的影响,探讨运动降压的机制。方法:原发性高血压大鼠随机分为对照组(SHR-C)和训练组(SHR-T),配对正常血压大鼠对照组(WKY-C)(n=7)。SHR-T进行10周游泳训练,每周训练5 d,每天运动1次,第1周每次运动40 min,第2周50 min,第3周增加到60 min后保持不变,直至训练完毕。SHR-T训练结束24 h后大鼠全部处死并提取比目鱼肌,RT-PCR和免疫印迹法测定血管内皮生长因子(VEGF)等指标。结果:与WKY-C比较,SHR-C骨骼肌VEGF、内皮型一氧化氮合酶(eNOS)蛋白明显低于对照组(P<0.05),训练前SHR-C、SHR-T两组血压显著性高于WKY-C(P<0.01);训练结束后,与SHR-C比较,SHR-T血压显著性降低(P<0.05),心率降低更加显著(P<0.01),VEGF mRNA及蛋白、VEGFR2、eNOS均显著性升高(P<0.05)。结论:氧运动训练能明显降低高血压大鼠血压,促进骨骼肌VEGF mRNA和蛋白水平的表达,同步提高VEGFR2、eNOS蛋白含量,血管生长因子水平增加有利于血管生成并产生降压效应。
Objective： To investigate effects of exercise training on vascular regulators and discuss its antihypertensive mechanism. Methods： Rats were divided into three groups（ n = 7）： spontaneous hypertensive rats control group（SHR-C）, training group（SHR-T） and nor- motensive wistar-kyoto control group（WKY-C）. Aerobic exereise consisted of 10 weeks of swimming training for 5 days/week. Exercise duration was 40 min in the first week, then 50 min in the second week, from the third week to the end of training, duration was maintained at 60 min. After training, vascular endothelial growth factor （VEGF） and other biomarkers in seleus were measured by RT-PCR and immunoblotting. Results： VEGF and endothelial NO synthase （eNOS） in SHR-C were lower than that in WKY-C（ P 〈0.05）. Blood pressure in SHR-C and SHR-T were higher than that in WKY-C before training; After training, compared with SHR-C, VEGFR2, eNOS, VEGF and VEGF mRNA increased significantly in SHR-T paralleled with marked decreases in blood pressure and heart rate respectively （ P 〈 0.05, P 〈 0.01）. Conclusion： Aerobic exercise training lowered the blood pressure in spontaneous hypertensive rats, and promoted VEGF mRNA level and expressious of VEGF, VEGFR2 and eNOS. The up-regulations of these vascular regulators could benefit angiogenesis and contribute to the antihyperteusive effects.
出处
《中国应用生理学杂志》
CAS
CSCD
2014年第4期320-324,共5页
Chinese Journal of Applied Physiology
