摘要
试验条件下研究了不同质量浓度(2 mg·L-1、8 mg·L-1和32 mg·L-1)双环芳烃——萘暴露对环文蛤(Cyclina sinensis)超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、碱性磷酸酶(AKP)活性和丙二醛(MDA)生成量变化的影响。结果表明,高浓度萘(32 mg·L-1)在15 d内对环文蛤有很强的致死效应;在非致死条件下,随暴露时间延长SOD活性持续升高,CAT活力存在低浓度促进高浓度抑制的现象,AKP活性随暴露时间延长表现出先升高后降低的趋势,而MDA生成量则随暴露时间延长呈现低→高→低的变化。Pearson相关分析显示,在环文蛤应对氧化胁迫的过程中SOD和CAT呈现协同作用(R=0.439,P<0.01),SOD和AKP表现拮抗关系(R=-0.571,P<0.01)。在保护细胞膜结构完整性方面,CAT和AKP可能较SOD发挥更大作用(R=-0.490,P<0.01)。
The SOD,CAT,AKP activities and MDA content were determined after Cyclina sinensis were exposed to different concen-trations（2 mg·L ^- 1 ,8 mg·L^ - 1 and 32 mg·L ^- 1 ）of naphthalene under experimental ecotoxicology conditions. The results indicate that C. sinensis had high mortality rate under 32 mg·L^ - 1 of naphthalene. Under non-lethal conditions and with increase of naphthalene expo-sure time,SOD activity showed a continuous increasing trend;CAT activity was promoted in low-concentration group and inhibited in high-concentration group;AKP activity was initially increasing then decreasing;MDA content fluctuated following the trend of low→high→low. According to Pearson correlation analysis,SOD and CAT showed synergistic effect（R = 0. 439,P ﹤ 0. 01）,while SOD and AKP showed antagonism effect（R = - 0. 571,P ﹤ 0. 01）. CAT and AKP might protect cellular membrane structure better than SOD（R = - 0. 490,P ﹤ 0. 01）.
出处
《南方水产科学》
CAS
CSCD
北大核心
2014年第4期39-44,共6页
South China Fisheries Science
基金
天津市科技兴海项目(KJXH2011-05)
天津农学院科学研究发展计划项目(2013NO8)
关键词
多环芳烃
萘
环文蛤
氧化胁迫
生化指标
polycyclic aromatic hydrocarbon
naphthalene
Cyclina sinensis
oxidative stress
biochemical indicators
