摘要
目的：建立测定青果丸中黄芩苷含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent TC-C18（150 mm×4.60mm,5μm）,流动相为甲醇-水-0.02 mol/l磷酸（45∶55∶0.2,V/V/V）,流速为1.0 ml/min,柱温为30℃,进样量为10μl,检测波长为278 nm。结果：黄芩苷检测质量浓度在10~200μg/ml范围内与峰面积积分值呈良好的线性关系（r=0.999 9,n=6）;精密度、稳定性、重复性试验的RSD≤0.96%;平均加样回收率为99.53%,RSD=1.07%（n=9）。结论：该方法简便、准确、重复性好,可用于青果丸中黄芩苷的含量测定。
OBJECTIVE： To establish a method for the content determination of baicalin in Qingguo pills. METHODS： HPLC method was adopted. The determination was performed on Agilent TC-C18（150 mm×4.60 mm, 5μm） column with mobile phase consisted of methanol-water-0.02 mol/1 phosphoric acid （45 ： 55 ： 0.2, V/F/V） at the flow rate of 1.0 mL/min. The column tempera- ture was set at 30 ℃ The sample size was 10μl, and detection wavelength was set at 278 nm. RESULTS： The linear range of baicalin was 10-200 μg/ml （r=0.999 9, n=6） with an average recovery of 99.55% （RSD=1.07%, n=9）; RSD of precision, stability and reproducibility tests were lower than 0.96%. CONCLUSIONS： The method is simple, accurate and reproducible, and can be used for the content determination of baicalin in Qingguo pills.
出处
《中国药房》
CAS
CSCD
2014年第32期3057-3058,共2页
China Pharmacy
