摘要
为了比较尿液蛋白PVDF膜富集保存法(尿膜)和尿液直接冻存法两种方法的优缺点。通过比较两种方法在时间、所占空间、费用、蛋白降解程度及大样本临床实践性方面的区别。发现在所占空间、电费方面及临床实践性方面尿膜保存法优于直接冻存法,而在时间及耗材花费方面直接冻存法优于尿膜保存法。因此尿蛋白的尿膜保存法比直接冻存法有更强的实际应用价值。
To compare two enrichment and preservation methods of urinary proteins, stored in polyvinylidene difluoride （PVDF） membrane （Urimem） or direct freezing, we examined the differences between the two methods in time, space, costs of supplies and electricity, degree of protein degradation and convenience of the sample handling. The urimem method is superior in the storage space, the cost of electricity and the clinical convenience compared to the direct freezing method. However, the direct freezing method is superior in the time and the cost of supplies to the urimem method. The enrichment and preservation of urinary proteins using urimem have more cost-effective benefits compared to those of the direct freezing method.
出处
《生物工程学报》
CAS
CSCD
北大核心
2014年第7期1128-1133,共6页
Chinese Journal of Biotechnology
基金
卫生公益性行业科研专项基金(No.200702008)
“十二五”国家科技支撑计划(Nos.2011BA110B00,2011BA110B05)资助~~
关键词
尿液蛋白
富集保存方法
成本效益分析
urine proteins, enrichment and preservation methods, cost-effective analysis
