期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

eIF4E和整合素αvβ6在结肠癌组织中的表达及临床意义 被引量：4

Clinical significance and their relationship of e IF4E and inte-grin αvβ6 protein expression in colon cancer
暂未订购
导出
摘要 目的:探讨e IF4E和整合素αvβ6在结肠癌组织中的表达及临床意义。方法:采用免疫组化SP法检测69例结肠癌及30例正常结肠组织中e IF4E和整合素αvβ6蛋白的表达,并分析二者与结肠癌临床病理因素的关系。结果:结肠癌组织中e IF4E和整合素αvβ6蛋白的表达明显高于正常结肠组织,差异有统计学意义(P<0.01)。e IF4E表达与淋巴结转移有关(P=0.013),整合素αvβ6表达与肿瘤浸润深度、淋巴结转移及临床TNM分期有关(P=0.027,P=0.034,P=0.031)。结肠癌组织中e IF4E与整合素αvβ6蛋白的表达呈低度正相关(P=0.016,r=0.288)。Kaplan-Meier法生存分析显示,e IF4E和整合素αvβ6蛋白的表达与患者5年生存率显著相关(P=0.008,P=0.040)。Cox回归分析显示,e IF4E及整合素αVβ6蛋白的表达水平、组织分化程度、肿瘤浸润深度和年龄是结肠癌的独立危险因素,其中e IF4E高表达和αVβ6阳性表达的相对危险度分别是4.212(95%CI:1.779,9.973)和2.774(95%CI:1.148,6.700)。结论:结肠癌组织中e IF4E和整合素αvβ6蛋白可作为结肠癌患者术后预后不良的独立预测指标,二者潜在的信号通路可能成为治疗结肠癌的新靶点。 Objective：To investigate the expression of elF4E and Integrin α v β 6,their clinical significance as well as two proteins＇ correlation in colonic carcinoma tissue.Methods：The expression of elF4E and integrin α v β 6 was detected in 69 colonic carcinoma tissues and 30 normal colon tissues via SP immunohistochemistry method.And the relationship between the two proteins＇expression and clinicopathological characteristics were analyzed.Results：The expression of elF4E and integrin αv β 6 in colon cancer tissue was significantly higher than that in normal colon tissue （P＜0.001）.The expression of elF4E was significantly associated with tumor metastasis,while integrin α v β 6 was related to invasion depth,tumor metastasis and clinical TNM stage （P=0.027,P=0.034,P=0.031 respectively）.Moderately weak correlation exists between the two proteins （P=0.016,P=0.288）.The survival analysis by Kaplan-Meier and Cox regression model showed that protein expression of high eIF4E and positive integrin αv β 6,as independent prognostic factors （RR=4.212,RR=2.774 respectively）,tended to have a significantly poorer 5-year survival rate（P=0.008,P=0.040 respectively）.Conclusion：elF4 E and integrin αv β 6 can be indicators of poor prognosis for patients with colon cancer.And the potential signaling loop involving them may provide a helpful therapeutic target in prevention and treatment of colon cancer.
作者 牛正川 张仁华 牛卫博 刘恩宇 彭程 贺兆斌 赵传宗 洪建国 高超 鲁志华 王加勇 牛军
机构地区 山东大学齐鲁医院肝胆外科
出处 《中国现代普通外科进展》 CAS 2014年第6期439-443,共5页 Chinese Journal of Current Advances in General Surgery
基金 国家自然科学基金(81272653) 山东省自然科学基金(ZR2013HQ051)
关键词 结肠肿瘤 翻译起始因子 整合素ΑVΒ6 Colonic neoplasms Eukaryotic initiation factor Integrins αvβ6
分类号 R735.35 [医药卫生—肿瘤]
  • 相关文献

参考文献21

  • 1Akagi Y, Adachi Y, Kinugasa T, et al. Lymph node evaluation and survival in colorectal cancer: review of population-based, prospec- tive studies[J]. Anticancer Res,2013,33:2839-2847.
  • 2Zimmer SG, DeBenedetti A, Graft JR. Translational control of ma- lignancy: the mRNA cap-binding protein, eIF-4E, as a central reg- ulator of tumor formation, growth, invasion and metastasis [J]. Anti- cancer Res, 2000,20:1343-1351.
  • 3Malgorzata Barczyk, Sergio Carracedo, Donald Gullberg[J]. Integrins Cell Tissue Res, 2010,339:269-280.
  • 4Chung J, Kim TH. Integrin-Dependent Translational Control: Impli- cation in Cancer Progression[J]. Microsc Res Tech, 2008,71:380-386.
  • 5Culjkovic B, Topisirovic I, Skrabanek L, et al. eIF4E is a central node of an RNA regulon that governs cellular proliferation[J]. J Cell Biol, 2006,175 (3):415-426.
  • 6Wang J, Wu J, Niu J. PKC promotes the migration of colon cancer cells by regulating the internalization and recycling of integrin αvβ6[J].Cancer Lett, 2011,311 ( 1 ):38-47.
  • 7Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN[J]. International Journal of Can- cer,2010,127 (12) :2893-2917.
  • 8Culjkovie B, Topisirovic I, Borden KL. Controlling gene expression through RNA regulons: the role of the eukaryotic translation initia- tion factor elF4E[J]. Cell Cycle, 2007,6 (1):65-69.
  • 9Arrigo De Benedetti, Jeremy R Graff. eIF-4E expression and its role in malignancies and metastases[J]. Oncogene, 2004,23:3189-3199.
  • 10Ruggero D, Montanaro L, Ma L, et al. The translation factor eIF- 4E promotes tumor formation and cooperates with c-Myc in lym- phomagenesis[J]. Nat Med, 2004,10:484-486.

二级参考文献27

  • 1曹耀辉,赵松,吴小华,王小玲.整合素αvβ6在卵巢癌中的表达和生物学意义[J].河北医科大学学报,2005,26(5):359-361. 被引量：3
  • 2杨广运,徐克森,张朝阳,王金申,王加勇,刘炎锋,帅晶,寿楠海,牛军.整合素ανβ6-ERK2直接通路调控MMP-9分泌与结肠癌转移关系的实验研究[J].中华普通外科杂志,2007,22(6):443-445. 被引量：8
  • 3Hynes RO. lntegrins: versatility, modulation, and signaling in cell adhesion[J]. Cell, 1992,69(1):11-25.
  • 4Sheppard D, Rozzo C, Starr L, et al. Complete amino acid sequence of a novel integrin beta subunit (beta 6) identified in epithelial cells using the polymerase chain reaction [J].J Biol Chem, 1990,265(20): 11502-11507.
  • 5Huang X, Wu J, Spong S, et al. The integrin alphavbeta6 is critical for keratinocyte migration on both its known ligand, fibronectin, and on vitronectin[J]. J Cell Sci, 1998,111(Pt15):2189-2195.
  • 6Munger JS, Huang X, Kawakatsu H, et al. The integrin alpha v beta 6 binds and activates latent TGF beta 1: a mechanism for regulating pulmonary inflammation and fibrosis[J]. Cell, 1999,96(3):319-328.
  • 7Sipos B, Hahn D, Carceller A, et al. Immunohistochemical screening for beta6-integrin subunit expression in adenocarcinomas using a novel monoclonal antibody reveals strong up-regulation in pancreatic ductal adenocarcinomas in vivo and in vitro [J]. Histopathology, 2004,45(3):226-236.
  • 8Ahmed N, Niu J, Dorahy DJ, et al. Direct integrin alphavbeta6- ERK binding: implications for tumour growth[J]. Oncogene, 2002,21 (9): 1370-1380.
  • 9Gu X, Niu J, Dorahy D J, et al. Integrin alpha(v)beta6-associated ERK2 mediates MMP-9 secretion in colon cancer cells [J]. Br J Cancer, 2002,87(3):348-351.
  • 10Ahmed N, Pansino F, Baker M, Rice G, et al. Association between alphavbeta6 integrin expression, elevated p42/44 kDa MAPK, and plasminogen-dependent matrix degradation in ovarian cancer [J].J Cell Biachem, 2002,84(4):675-686.

共引文献2

同被引文献27

  • 1Akagi Y,Adachi Y,Kinugasa T,et al.Lymph node evaluation and survival in colorectal cancer:review of population-based,prospective studies[J].Anticancer Res,2013,33(7):2839-2847.
  • 2Ferlay J,Soerjomataram I,Dikshit R,et al.Cancer incidence and mortality wordwide:sources,methods and major patterns in GLOBOCAN 2012[J].Int J Cancer,2015,136(5):e359-386.
  • 3Bonnans C, Chou J, Werb Z. Remodelling the extracellular ma- trix in development and disease [J]. Nat Rev Mol Cell Biol, 2014,15(12) : 786-801.
  • 4Harisi R,Jeney A. Extracellular matrix as target for antitumor therapy[J]. Onto Targets Ther, 2015,9(8) : 1387- 1398.
  • 5Sundquist E, Renko O, Salo S, et al. Neoplastic extracellular matrix environment promotes cancer invasion in vitro[J]. Exp Cell Res, 2016,344(2) :229-240.
  • 6Rubinstein B, Pinto IM. Epithelia migration: a spatiotemporal inter- play between contraction and adhesion[J]. Cell Adh Migr, 2015,9 (5) :340-344.
  • 7Jung HY,Fattet L,Yang J. Molecular pathways: linking tumor microenvironment to epithelial-mesenchymal transition in metas- tasis[J]. Clin Cancer Res,2015,21(5) :962-968.
  • 8Greenburg G, Hay ED. Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchy- mal cells [J]. J Cell Biol,1982,95(1):333-339.
  • 9Bates RC,Bellovin DI,Brown C,et al. Transcriptional activation of integrin beta6 during the epithelial-mesenchymal transition de- fines a novel prognostic indicator of aggressive colon carcinoma [J]. J Clin Invest,2005,115(2) .. 339-347.
  • 10Paz H,Pathak N,Yang J. Invading one step at a time: the role of invadopodia in tumor metastasis[J]. Oncogene,2014,33(33) 4193- 4202.

引证文献4

二级引证文献22

中国现代普通外科进展
2014年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部