摘要
目的 :观察骨髓间充质干细胞(BMSC)移植联合外源性VEGF-C蛋白治疗兔肢体淋巴水肿疗效。方法:手术和放射相结合制作兔后肢淋巴水肿模型,随机分为对照组(生理盐水组)、骨髓间充质干细胞治疗组(BMSC组)、VEGF-C治疗组(VEGF-C组)及联合治疗组(BV组),每组各8只。治疗后28 d测量肢体体积变化,并于治疗后30 d Western blot检测移植组织中VEGF-C表达、VEGFR-3免疫组化染色及微淋巴管计数。结果:Western blot结果显示,对照组、BMSC组及VEGF-C组的VEGF-C表达无显著性差异(P>0.05),BV组的VEGF-C表达较其余3组增多(P<0.05),对照组、BMSC组及VEGF-C组的微淋巴管计数组间比较差异无显著性(P>0.05),BV组的微淋巴管计数较其余3组明显增加(P<0.05)。BMSC组及VEGF-C组的VEGFR-3免疫组化染色呈阳性表达,与对照组比较有显著性差异(P<0.05),BV组的VEGFR-3免疫组化染色呈强阳性表达,与其余3组比较均有显著性差异(P<0.05)。结论:骨髓间充质干细胞联合VEGF-C可以更加有效地改善肢体淋巴水肿。
Objective：To determine the effect of bone marrow stromal cells transplantation and VEGF-C administration as a treatment for secondary lymphedema.Methods：BMSCs with/or VEGF-C protein were injected into lymphedema limbs.Water displacement volumetry was performed to measure limb volume changes.Immunohistochemistry was performed to detect VEGFR-3 and to count lymph vessel.Western blot analysis was performed to detect VEGF-C.Results：Before treatment,rabbits had average volume of edema in the limb of （62.23 ± 5.11）ml,（61.45 ± 6.15） ml,（60.79 ± 8.34）ml and （61.67 ±4.57）ml respectively in four groups.With therapy,this was reduced to an average volume of （26.53 ± 4.33）ml,（35.26 ± 5.57）ml,（37.43 ± 5.72）ml and （56.42 ± 6.93）ml respectively in four groups 28 days after treatment.Quantitative analysis showed that the vessel numbers were significantly increased in the BMSC＋ VEGF-C group,the BMSC group and the VEGF-C groups compared with the control group at 28 days after the operation （P＜0.05）.Western blot analysis demonstrated expression of VEGF-C was higher in BMSC＋ VEGF-C group and the BMSC group.Conclusion：The combined treatment with BMSCs transplantation and VEGF-C administration is superior to BMSCs transplantation alone in the treatment of limb lymphedema.BMSCs transplantation and VEGF-C administration could enhance the therapeutic effect of each other.
出处
《中国现代普通外科进展》
CAS
2014年第6期426-429,共4页
Chinese Journal of Current Advances in General Surgery
基金
山东省自然科学基金课题(ZR2012HM063)
山东大学大学生科技创新基金课题(201310422093)
