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柽柳ThERF1与其他ERF家族成员的互作

Family Interaction of ERF Tamarix hispida ThERF1 with Other Members
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摘要 通过对柽柳转录组数据进行Blast分析,得到7个与ThERF1同源的基因(ThERF4、ThERF7、ThERF11、ThERF12、ThERF13、ThERF14和ThERF16)。利用ClustalX V2.0对ThERF1和7个与其同源的基因的AP2结构域进行多序列比对,显示了这8个ThERF基因保守区之间的相似关系。酵母双杂交结果表明,ThERF1能与其自身互作,还能与ThERF4、ThERF11、ThERF16互作,说明ThERF1能够与自身或其家族成员形成同源或异源二聚体来行使功能,进而为深入研究ThERF1的耐盐机制奠定了基础。 We obtained seven ERF genes by Blast analysis on the transcriptoms from Tamarix hispida, including ThERF4, ThERF7, ThERF11, ThERF12, ThERF13, ThERF14 and ThERF16.By mutiple sequence alignments , ThERF1 shared high similarities in the AP 2 domain with seven ERFs .By yeast two-hybrid analysis , ThERF1 can interact with itself and al-so interact with ThERF4, ThERF11 and ThERF16, and it can work in the forms of homodimers with itself or heterodimers with other ERFs .
作者 聂显光 及晓宇 刘羽佳 刘文进 王玉成
机构地区 林木遗传育种国家重点实验室(东北林业大学)
出处 《东北林业大学学报》 CAS CSCD 北大核心 2014年第8期6-9,24,共5页 Journal of Northeast Forestry University
关键词 柽柳( TAMARIX hispida) 酵母双杂交 互作蛋白 二聚体 Tamarix hispida Yeast two-hybrid Interacting protein Dimers
分类号 Q943.2 [生物学—植物学]
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参考文献21

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东北林业大学学报
2014年 第8期

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