摘要
目的 探讨低氧对心肌细胞血管内皮生长因子 (VEGF)表达的影响。方法 采用Kuwabara等并加以改进的方法对心肌细胞进行低氧培养 ,并于低氧培养的即刻、2 4、4 8h分别测定培养液中的氧分压。使用逆转录聚合酶链反应 (RT PCR)、免疫组织化学和酶联免疫吸附测定 (ELISA)法分别检测低氧培养的不同时间点VEGFmRNA及VEGF蛋白的表达和分泌。结果 与常规培养相比 ,简易低氧培养培养液氧分压降至 5 8mmHg ,并于 3个时间点保持这一水平 (P >0 .0 5 )。从mRNA水平和蛋白水平我们检测到VEGF表达的增加 ,于低氧培养 2 4h达峰值 ,培养液中的峰值浓度为 (72 4 .6 7± 5 6 .87)pg ml。结论 低氧促进心肌细胞表达VEGFmRNA。
Objective To study the effect of hypoxia on the vascular endothelial growth factor (VEGF)expression in cardiomyocytes.Methods Cardiomyocytes were cultured in hypoxic condition.PO 2 of cultured medium was tested at 0,24 and 48 hours after hypoxia.VEGFmRNA and its protein were examined respectively by RT PCR,immunohistochemistry and ELISA at different time points of hypoxic culture.Results PO 2 was about 58 mmHg in hypoxic culture and continued 48 hours(P>0.05).The expression of VEGF was increased at the levels of mRNA and protein,and reached a peak at 24 hour of hypoxic culture.The peak level of VEGF in culture medium was (724.67±56.87) pg/ml.Conclusion Hypoxia promotes the expression and secretion of VEGF in cultured cardiomyocytes.
出处
《中华老年心脑血管病杂志》
CAS
2002年第4期266-269,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
湖北省九五科技攻关项目 (962 9110 1)
