摘要
为了解某集约化猪场伪狂犬病病毒(PRV)的感染情况,清除感染猪只,净化伪狂犬病,提高猪群的健康水平。采用PRV gE抗体ELISA检测试剂盒,对该场免疫了猪伪狂犬病基因缺失疫苗的猪群进行3次野毒感染检测;应用荧光PCR方法,对临床发病的仔猪进行PRV病原检测。经过动态监测、加强综合防控和逐步淘汰措施,该猪场的PRV野毒感染率从35.29%逐步降低至0%,猪场自繁的仔猪存活率高,无PRV感染,净化效果良好。
In order to study the pseudorabies virus infection in intensive pig farm, eliminate the infected pigs, and guarantee the herd health,PRV gE antibody ELISA test kit was used to detect three batches ofsera from pigs which were immunized with gene deleted pseudorabies vaccine. At the same time, the fluorescence quantitative PCR method was used to detect the pathogen in the nosogenic piglets. The pseudorabies pathogen positive piglets were isolated so as to ensure the overall stability of pig farm. The results showed that by measures of dynamic monitoring, control and elimination method, the PRV wild virus infected rate had been reduced to 0% from 35.29%. Piglets survival rate was high with no swine pseudorabies, preliminary decontamination effect is attained.
出处
《广东畜牧兽医科技》
2014年第4期15-17,共3页
Guangdong Journal of Animal and Veterinary Science
关键词
伪狂犬病野毒
感染
检测
净化
Pseudorabies wild virus, ELISA, Detection of antibody, Decontamination
