摘要
目的:明确骨髓间充质干细胞(MSCs)对内毒素血症小鼠的治疗作用。方法:动物分为4组:对照组、内毒素血症组、间充质干细胞治疗组、间充质干细胞组。分别在内毒素脂多糖(LPS)注射24 h和7 d后观察小鼠心功能的变化,ELISA方法检测血清细胞因子的水平,组织学方法观察对心肌、肝脏、肺脏和肾脏形态学改变的影响。结果:与对照组小鼠相比,内毒素血症组经LPS刺激后血清白介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)含量增高,间充质干细胞治疗组血清IL-1β和TNF-α含量明显降低;内毒素血症组小鼠心功能明显下降,间充质干细胞治疗组心功能明显恢复;内毒素血症小鼠心肌细胞和肝细胞凋亡增加,肺间质和肺泡水肿,间充质干细胞治疗组上述组织损伤明显改善。结论:MSCs移植抑制了内毒素血症小鼠的炎症反应,改善了心功能,减轻了对心脏、肝脏和肺脏的损害。
Objective：To determine the effects of bone marrow mesenchymal stem cells on mice with endotoxemia. Methods：Mice were divided into the following groups：the control group,the endotoxemia group（the LPS group）,the mesenchymal stem cell treatment group（the LPS＋MSCs group） and the mesenchymal stem cell group（the MSCs group）. The cardiac function of mice was respectively observed at 24 hours and day 7 after LPS administration. ELISA was performed to detect the level of cytokines in the serum,and histological examinations were performed to detect morphological changes of the cardiac muscle,liver,lung and kidney. Results：Compared to the control group,serum IL-1β and TNF-α were increased in the mice of the LPS group,and MSCs treatment decreased serum IL-1β and TNF-α obviously. The cardiac function of the LPS group mice deteriorated,which was rescued by MSCs treatment.Apoptosis of cardiomyocytes and hepatocytes in the mice of the LPS group was increased. Edema was observed in the pulmonary interstitium and alveolus,and ameliorated by MSCs treatment. Conclusion：MSCs inhibit the inflammatory reaction of mice with endotoxemia,improves the cardiac function and reduces the damage of the heart,liver and lung.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第7期921-925,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省科技厅基础研究计划(BK20131449)
