摘要
目的了解深圳市家禽肉流通各环节的空肠弯曲菌污染情况,探讨空肠弯曲菌的分子分型特征。方法采集活家禽肛拭子、宰杀环境拭子、零售禽肉样本,婴幼儿腹泻患者粪便样本。依据GB/T4789.9-2008《食品卫生微生物学检验》进行检验。分离的菌株利用PFGE进行分型。结果 60份鸡肛拭子共检出空肠弯曲菌22株,阳性率为36.67%;30份鸭肛拭子共检出空肠弯曲菌3株,阳性率为10.00%;40份鹅肛拭子共检出空肠弯曲菌7株,阳性率为17.50%。12份脱毛机内壁拭子、6份案板拭子、12份工作人员手拭子,分别检出空肠弯曲菌2、1、1株。采集市场现宰新鲜禽肉40份,检出空肠弯曲菌7株,阳性率为17.50%;超市采集冷冻禽肉36份,未检出空肠弯曲杆菌。婴幼儿腹泻样本163份,检出空肠弯曲菌4株,检出率为2.45%。脉冲场凝胶电泳分析结果显示,分离得到的24株菌可分为24个型别。结论深圳地区禽肉食品各流通环节存在较普遍的空肠弯曲菌污染,婴幼儿感染空肠弯曲菌的状况也较常见。
Objective To investigate the contamination of poultry with Campylobacter jejuni（C.jejuni）in Shenzhen and to analyze the molecular characteristics,as well as to understand the status of its infection in infants and young children. Methods Anal swabs from live poultry,swabs from slaughter environment,meat samples from retail poultry and stool samples from infants and young children with diarrhea were collected and tested according to GB/T4789.9-2008 ＂ microbiological examination of food hygiene＂.C.jejuni bacteria were isolated and then typed by PFGE. Results The positive rate of C.jejuni was 36.67%（22/60）in chicken anal swabs,10.00%（3/30）and 17.50%（7/40）in duck and goose anal swabs.There were only two samples found positive in 12swabs collected inside the hair removal machine wall.One in 6cut board swabs and one in 12hand swabs was positive for C.jejuni.Out of 40fresh poultry meat samples,7were positive with the positive rate of 17.50%.None of the 40samples of frozen poultry meat was found positive.Meanwhile,the positive rate was 2.45%in 163stool samples collected from infants and young children with diarrhea.Pulsed field gel electrophoresis analysis showed that 24strains isolated were identified as 24types. ConclusionsThe contamination of poultry with Campylobacter jejuni is common in Shenzhen,as is the infection among infants and young children.
出处
《中国预防医学杂志》
CAS
2014年第4期353-356,共4页
Chinese Preventive Medicine
基金
深圳市科技计划项目(200802134)
关键词
空肠弯曲菌
污染
禽类
腹泻
Campylobacter jejuni
Contamination
Poultry
Diarrhea
