摘要
建立了一种采用超高效合相色谱分析卷烟烟气中8种重要羰基化合物的方法。使用衍生化试剂处理过的滤片捕集卷烟烟气中的重要羰基化合物,经乙腈萃取后,采用配备紫外检测器的合相液相色谱进行定量分析。使用SPSS软件对22个卷烟样品采用该方法和标准方法测定的数据进行配对t检验。结果表明:18种羰基化合物在0.02~10.00 mg/L范围内线性关系良好(R2>0.999),加标回收率在75%~96%之间,平均相对标准偏差小于10.0%,检出限为0.07~0.15μg/支。2与标准方法相比,该方法检测时间显著缩短。3两种方法所得数据的相关系数为0.938,显著性概率为0.082,大于0.05,两组数据没有显著差异。4该方法的流动相主要为二氧化碳,有机溶剂消耗极少,更符合绿色分析要求,并可降低分析成本。
An ultra performance convergence chromatography （UPC2） was developed for analyzing eight important carbonyl compounds in cigarette smoke, wherein the filter pads for trapping the said analytes were treated with derivative reagent. After extracting with acetonitrile, the said analytes were quantitatively analyzed by UPC2 equipped with UV detector. Twenty-two cigarette samples were parallel analyzed by this method and the standard method, the obtained data were compared by SPSS software via paired t test. The results showed that： 1） All of the eight carbonyl compounds displayed good linearity （R2〉0.999） in the range of 0.02-10.00 mg/L. The standard addition recoveries ranged from 75% to 96% with the relative standard deviation less than 10.0%. The limits of detection ranged from 0.07 to 0.15 μg/cigarette. 2） The detection time of this method was significantly shorter than that of the standard method. 3） There was no significant difference between the data obtained by the two methods with the correlation coefficient of 0.938 and the significance probability of 0.082 （〉 0.05）. 4） By taking carbon dioxide as mobile phase, this method consumed less organic solvent and was more environment-friendly, the cost of analysis reduced accordingly.
出处
《烟草科技》
EI
CAS
北大核心
2014年第7期51-55,共5页
Tobacco Science & Technology
基金
中国烟草总公司2013年度科技重点项目"国内外卷烟主要有害成分释放量中位值及其影响因素研究"(110201302008)
关键词
卷烟
主流烟气
羰基化合物
超高效合相色谱
配对T检验
Cigarette
Mainstream smoke
Carbonyls
Ultra performance convergence chromatography （UPC2）
Paired t test
