摘要
本研究设计建立了用一种酶标记抗体检测人和不同种属动物鼠疫血清抗体的 ELISA抑制法系统,其主要步骤用抗 Fl 兔 IgG 包被微板、加入用1单位 Fl 抗原液稀释的被检血清,再加入抗 Fl 兔 IgG 酶标记物、1单位 Fl 抗原液为对照,以抑制率≥50%为阳性,阳性临界滴度为1∶20。该法通过鼠疫疫区9份疑似鼠疫病人血清和580份动物血清的检测结果表明,无论从检出率或抗体水平均显著高于 PHA 法(P<0.01),并且未出现1份假阳性反应血清,而 PHA 却存在3.8%～17.6%假阳性反应。该检测系统不仅具有用一种酶标记抗体能有效的检测人和不同种属动物的血清抗体,而且敏感性好,特异性强,操作简便,结果重复性好,为鼠疫和其它人兽共患病血清抗体的检测,提供了一种优于常规 ELISA 和 PHA 的检测手段。
In present study a method of Inhib-ELISA system was established using theenzyme-liked antibodies to examine the serum antibodies of the plague in humanand various kind of animals.The serum 9 individuals who were taken from in the plague epidemic areassuspected as the plague and 580 serum samples of animals were detected withthis method.The results showed that the detecting rate and the levels of antibodieswere significantly higher than that of PHA method(P<0.01)and there was nonof the serum samples shower a false positive result.This detective system couldnot only effectively examine the serum antibodies of man and various kind ofanimals with one kind of enzyme linked antibody,but also have a high sensitiv-ity and specificity.As this measure was simple and the results were reproduceableit would be a better method for detecting serum antibodies of the plague orzoonosis than routine ELISA and PHA examination.
出处
《中国地方病学杂志》
CAS
CSCD
1991年第6期321-324,共4页
Chinese Jouranl of Endemiology
关键词
鼠疫
人兽共患病
ELISA
plague
serologic investigation of zoonosis
