摘要
为了观察甲状腺调节因子如细胞因子、激素及神经递质对甲状腺细胞(TEC)细胞间粘附分子-1(ICAM-1)表达的影响,本研究在细胞培养基础上,采用ELISA法检测不同因子刺激后细胞上清液中可溶性ICAM-1(sICAM-1)的含量。结果表明:(1)细胞因子IL-1、IL-6可显著抑制TEC sICAM-1的合成,而TNF-α和IFN-γ则明显刺激sICAM-1产生;(2)肾上腺素、去甲肾上腺素、乙酰胆碱均可显著抑制TEC分泌sICAM-1;(3)促甲状腺素和地塞米松可显著抑制sICAM-1的生成;而泌乳素和雌激素则明显促进sICAM-1的产生。提示甲状腺调节因子可通过影响ICAM-1表达,参与甲状腺局部免疫调节过程。
To investigate the effects of various thyroid regulatory factors, such as cytokines, neurotransmitters or hor-mones on the expressions of ICAM-1 on thyrocytes of patients with Graves disease, thyrocytes from patients were cultured in the presence or absence of the thyroid regulatory factors, and the soluble ICAM-1 (sICAM-1 ) in the super-natants of cultured cells were detected by ELISA. The results showed that the expression of sICAM-1 on the thyrocytes of patients was markedly inhibited by IL-1 and IL-6, but increased by TNF-a and IFN-r. Epinephrine, norepinephrine and acetylcholine could significantly reduce the secretion of sICAM-1 from thyrocytes. The production of sICAM-1 from the thyrocytes of patients was markedly inhibited by TSH and dexamethasone, but enhanced by prolactin and estrogen. It concludes that thyroid regulatory factors play an important role in the regulations of local immune responses of thyroid gland through modulation of ICAM-1 expression.
出处
《上海免疫学杂志》
CSCD
北大核心
2002年第4期273-274,277,共3页
Shanghai Journal of Immunology
