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体内预变性坐骨神经体外培养雪旺细胞 被引量:1

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摘要 目的 介绍一种快速、简便获取大量Schwann细胞培养方法。方法 体内切断一侧坐骨神经,1、2、3、4d及1、2周后取坐骨神经进行体外Schwann细胞培养。对侧不做任何处理的坐骨神经作为对照组。同时在培养液中加入刺激因子forskolin和BTI。结果 体内预变性后Schwann细胞产量高于对照组3倍;加刺激因子forskolin和BTI后高于对照组5倍。预变性的最佳时间为1周。结论 这种体内预变性坐骨神经后再进行体外Schwann细胞培养不失为一种较好的实验方法。
出处 《中华国际医学杂志》 2001年第2期98-100,共3页
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