摘要
目的 观察分次外照射对小细胞肺癌NCI H4 4 6细胞系总RNA及其MDR1mRNA的影响。方法 采用GWGP80型远距离60 Co治疗机对进入指数生长期的NCI H4 4 6小细胞肺癌细胞系进行分次外照射 ,每次2Gy ,总剂量 5 0Gy。用Trizol分别提取未照射和已完成全部外照射剂量NCI H4 4 6细胞系的总RNA。通过RT PCR、琼脂胶电泳、Gelbase电脑软件计算电泳条带的平均吸光度A值 ,用两组细胞MDR1DNA与其 β actinDNA的平均吸光度A值的比确定两组细胞MDR1mRNA表达的高低。结果 在相同细胞数和相同体积的条件下 ,未照射组和照射组细胞总RNA的浓度分别为 2 5 .9μg/ml和 16 .6 μg/ml;未照射组细胞其MDR1DNA/ β actinDNA为1.0 78,照射组为 1.338。结论 对小细胞肺癌细胞系NCI H4 4 6进行外照射可抑制其总RNA的生物合成 。
Objective To investigate the effect of fractioned radiation treatment on the total RNA and MDR 1gene of NCI H446 small cell lung cancer cell line.Methods Exponentially growing NCI H446 cells were exposed to 50 Gy radiation administered in 25 fraction,at 2 Gy per fractions.The total RNA of cells that were irradiated and unirradiated was isolated from homogenate samples by the acid guanidine thiocyanate phenol chloroform method.The expression of MDR 1 gene was assessed by semi quantitative RT PCR assay.Gelbase software was used to calculate average OD value of every electrophoresis trip,and the average OD values ratio of MDR 1DNA and its β actin DNA of the two kinds of cells were acquired.The larger ratio indicate the increase of MDR 1 DNA expression.Results To the unirradiated cells,the concentration of total RNA was 25.9μg/ml and the ratio of the average value of electrophoresis trip from MDR 1DNA/β actin DNA was 1.078,but to the irradiated cells,these two numerical values were 16.6μg/ml and 1.338 respectively.Conclusions To exert radiation treatment to the NCI H446 small cell lung cancer cell line may inhibit the synthesis of its total RNA and enhance the expression of its MDR 1 gene at the same time.
出处
《北京医学》
CAS
北大核心
2002年第4期254-256,共3页
Beijing Medical Journal
