摘要
目的 探讨临床检测解脲支原体的实用方法。方法 对 60例尿道炎、阴道炎患者的尿道或阴道分泌物同时作解脲支原体培养和多聚酶链反应 (PCR)法检测 ,再将培养阳性的阳性产物作PCR法复核。结果 60例患者 ,培养法的阳性率为 45 % (2 7 60 ) ,PCR法阳性率为 41 .7% (2 5 60 )。 2种方法阳性率无显著性差异 ;2 7例培养阳性产物作PCR复检有 2 3例阳性 ,此 2 3例与 2种方法均阳性病例相同。
Objective To explore a practical method for detecting Ureaplasma urealyticum (UU) in clinic.?Methods Two techniques including culture and polymerase chain reaction (PCR) were used to detect UU in the secretion from 60 patients with urethritis or vaginitis, and the positive products of culture were checked by PCR.?Results The positive rate of UU was 45% (27/60) in culture and 41.7% (25/60) in PCR. No significant differences were found between the two methods. 23 out of the 27 culture positive cases were positive to PCR.?Conclusion It is feasible to use PCR technique to detect UU in clinic.
出处
《徐州医学院学报》
CAS
2002年第4期300-301,共2页
Acta Academiae Medicinae Xuzhou
