摘要
应用免疫组织化学方法观察抗呆Ⅰ号对小鼠前脑缺血再灌注损伤海马星形胶质细胞表达bcl 2和bax动态变化的影响 ,探讨其与缺血性神经元的联系。结果发现 :前脑缺血再灌注 3～ 7dbcl 2表达呈强阳性。再灌注 7～ 10dbax表达呈强阳性。用药组bcl 2和bax的表达较模型组均减弱。而正常对照组和假手术组无bcl 2和bax的表达。且星形胶质细胞表达bcl 2和bax的强弱与神经细胞的存亡有关。本实验提示 :前脑缺血再灌注后海马反应性星形胶质细胞表达bcl 2和bax呈动态变化 ,抗呆Ⅰ号对缺血再灌注损伤星形胶质细胞和神经细胞有良好的保护作用。
The effects of Kangdai No.1 on the dynamic changes in bcl 2 and bax expressions in hippocampal astrocytes in the mice with the cerebral injury caused by transient forebrain ischemia and reperfusion, and the relationship between the effects of Kandai No.1 and ischemic neurons were investigated by the immunohistochemical method. The results showed that bcl 2 expression was positive in 3 to 7 days after ischemia and reperfusion and bax expression was positive in 7 to 10 days after ischemia and reperfusion in the animals in the model group; both bcl 2 and bax expressions were weakened in the animals in the Kangdai No.1 treated group compared to those in the model group; there were no bcl 2 and bax expressions in the normal control group and sham operation group; and the intensity of bcl 2 and bax expressions in the astrocytes was related with the survival state of neurons. The results indicated that there were dynamic changes in bcl 2 and bax expressions in hippocampal reactive astrocytes after forebrain ischemia and reperfusion, and Kangdai No.1 had good protective effects against the injury of astrocytes and neurons caused by forebrain ischemia and reperfusion.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2002年第4期38-41,共4页
Journal of Beijing University of Traditional Chinese Medicine
