摘要
以8只犬形成自身对照的心肌缺血模型:自左前降冠脉和左旋冠脉随机选出两个基本一致的分支并同时结扎30分钟,以同样灌注压力和速度分别输入辅酶Q_(10)及生理盐水,共2小时。每只犬心肌分成三个区域:1):给药区;2) 盐水对照区;3) 正常区。实验结束时分别从三个区域取材测定MDA含量、NAG酶活性,线粒体横截面积、密度及此表面、局部心肌收缩力、心肌缺血和坏死区面积。结果:给药区MAD含量、NAG酶活性、线粒体横截面积和心肌坏死量、心肌细胞损伤程度明显低于盐水对照区,(MDA含量:nmol/100mg·pro 278.37±51.07,203.08±36.67和633.06±93.47,P<0.01;NAG酶;U/g0.19±0.02,0.17±0.04和0.38±0.06,P<0.05;心肌坏死量:18±2%和30±1%)而局部心肌收缩力和线粒体密度等明显高于盐水对照区,提示辅酶Q_(10)对缺血心肌具有保护作用。
Eight dogs were made a comparing model by itself. The two branches were selected from the left anterior descending coronary artery and/or the left circumflex coronary artery and were occluded for 30 minutes, then the two branches were randomly perfused for 2 hours with coenzyme Q_(10) and normal saline respectively. The myocardium of each dog was divided into three areas: 1). Coenzyme Q_(10) (0.6mg/kg); 2). Normal saline; 3). Nonischemic. Subsequently myocardium samples were taken from the 1, 2 and 3 areas to measure MDA, NAGase contents, cross-section areas, density of the mitochondrion, regional myocardial tension, the size of ischemia and necrotic mass. The results showed: MDA, NAGase were decreased in 1st and 3rd areas comparing to 2nd areas (MDA: nmol/100mg, pro 278.37±51.07,203.08±36.67 and 633.06±93.47, P<0.01; NAGase: U/g 0.19±0.02, 0.17±0.04, and 0.38±0.06, P<0.05, respectively.); the cross-section areas of the mitochondria and myocardial necrotic mass were larger in 2nd areas than 1st areas (30±1% and 18±2%, P<0.01, respectively); regional myocardial tension and mitochondrial density were less in 2nd areas than 1st areas. It is suggested that the coenzyme Q_(10) have protective effects on the ischemic myocardium.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1991年第1期38-41,共4页
Chinese Journal of Pathophysiology
关键词
心肌缺血
液体负荷
心肌
辅酶Q
Coenzyme Q
Myocardial contraction
Heart mitochondria
