摘要
本实验用甲基纤维素复盖层的c_6/36单层细胞微量蚀斑法的间接免疫荧光法,初步观察了登革热Ⅱ型病毒与靶细胞的联接反应情况。同时还制备了四种细胞的可溶性膜制剂,并研究了它们对登革热Ⅱ型病毒的抑制活性。结果提示,对登革热Ⅱ型病毒敏感的细胞表面存在登革热Ⅱ型病毒受体,在触发病毒感染阶段起重要作用。
To investigate the role of viral receptor in the initiation of dengue virus infection, viral attachment rates (AR) on Aedes albopictus cell (C6/36), human monocyte-like cell (U937), human T4 (H9),B lymphoblastoid cell(Raji), mouse myeloma cell (SP2/0) and mouse fibroblast cell (L929) were calculated by measuring the residual virus concentration after inoculating a known dose of Den-2 virus upon the above 6 cell lines and then keeping the cells at 4℃ for 15, 30 and 90 mino.The results showed that the ARs of Den-2 on C6/36, H9 and U937 were much higher than the ARs of Den-2 on L929, SP2/0 and Raji cells, the difference being highly significant (P<0.001). Solubilized cellular membrane preparations(SCM) were prepared by ultrasonic disintegration followed by ultracentrifugation and sodium deoxycholate treatment of C6/36, U937, L929 and Raji cells. Definite inhibitory actions upon Den-2 infectivity were shown in the SCM from C6/36 and U937 cells after their interaction with Den-2 at 4℃ for 4 hour, suggesting the SCM contained specific molecular complex closely related with Den-2 receptor and capable of blocking the infectivity of Den-2 virus. Only very weak inhibition of Den-2 infectivity could be found in the SCMs from L929 and SP2/0 cells. The above findings suggest the existence on the surface of susceptible cells Den-2 virus receptors which might play a crucial role in the initial step of virus infection.
出处
《中国病毒学》
CSCD
1991年第3期187-192,共6页
Virologica Sinica
关键词
登革热病毒
Ⅱ型
受体
Dengue-2 virus Attachment assay Solubilized cellular membrane (SCM) Virus receptor
