摘要
目的 :探讨DNA拓扑异构酶Ⅱ (TopoisomeraseⅡ ,TopoⅡ )及P 糖蛋白 (P glycoprotein ,P gp)在正常宫颈组织、宫颈上皮内瘤变 (cervicalintraepithelialneoplasm ,CIN)和宫颈癌中的表达。方法 :采用免疫组化的方法对 18例正常宫颈组织、2 8例CIN及 5 8例浸润性宫颈癌进行TopoⅡ和P gp的检测。结果 :TopoⅡ在正常宫颈组织、CIN及宫颈癌中均有表达 ,但在CINⅢ和宫颈癌中表达水平高于正常组织和CINⅠ组。P gp在正常宫颈组织中无阳性表达 ,在CINⅠ、CINⅡ、CINⅢ组中表达水平明显高于浸润性宫颈癌组。TopoⅡ的表达与浸润性宫颈癌组织学分级无关 ,而P gp在低分化癌的阳性率明显低于中高分化癌。结论 :TopoⅡ是检测肿瘤细胞增殖的一种免疫标记物 ,其在宫颈癌中明显高表达 ,选择TopoⅡ抑制剂进行化疗有助于提高化疗效果。P
Objective To investigate the expression of topoisomeraseⅡ(TopoⅡ) and P glycoprotein(P gp) in normal cervical squamous tissue,cervical intraepithelial neoplasm(CIN) and cervical squamous carcinoma.Methods Immunohistochemical method was used to detect the expression of TopoⅡ and P gp in 18 cases of normal cervical tissue,28 cases of CIN and 58 cases of invasive cervical carcinoma.Results Normal cervical tissue,CIN and invasive carcinoma all expressed TopoⅡ.And the positive expression in invasive cervical carcinoma and CINⅢ was higher than that in normal tissue and CINⅠ.There is no expression of P gp in normal tissue,and in CINⅠ,CINⅡ,CINⅢ P gp expressed higher than that in invasive cervical carcinoma. The expression of TopoII did not correlate with the differentiation,but the expression of P gp was lower in poorly differentiated carcinomas than that in well differentiated ones( P <0 05).Cordusions Topoisomerase Ⅱ is a useful proliferation marker in the detection of cervical squamous carcinoma.TopoⅡ was expressed highly in invasive cervical carcinoma and could be the target of anti tumor drugs,so TopoⅡ inhibitors should be selected to improve the efficiency of chemotherapy.The expression rate of P gp may not play an important rale in the drug resistance in cervical squamous carcinoma.
出处
《肿瘤防治杂志》
2002年第4期395-397,共3页
China Journal of Cancer Prevention and Treatment
基金
湖北省卫生厅资助项目 (WJ0 15 89)
