摘要
目的 观察秋水仙碱对肾间质纤维化的保护作用。方法 2 6只SD大鼠随机被分成 3组 :假手术组 (n =6 ) ;对照组 (n =10 ) ;治疗组 (n =10 ) ,单侧输尿管结扎 (UUO)基础上予秋水仙碱10 0 μg/(kg·d)腹腔注射 ,每周 5天。 3周后处死所有大鼠并进行形态学研究。 结果 治疗组肾小管间质浸润的炎症细胞数为 (1738 4 4± 910 87)个 /mm2 ,明显少于对照组 (6 32 2 0 5± 1198 18)个 /mm2 ,(P <0 0 0 1) ;肾间质纤维化指数前者也明显低于后者 (分别为 2 2 6 7± 17 6 1及 72 92± 9 86 ,P <0 0 0 1) ;肾小管损伤程度对照组和治疗组分别为 (2 4 7± 0 4 7)分和 (1 4 3± 0 6 5 )分 ,后者明显轻于前者 (P <0 0 5 )。结论 秋水仙碱对肾间质纤维化有较好的防治作用。
Objective To observe the effects of colchicine on renal interstitial fibrosis in rats with unilateral ureteral obstruction (UUO).Methods 26 Sprague Dawley rats were divided into three groups:the colchicine treated group (10 rats):UUO+colchicine 100μg/(kg·d) by intraperitoneally(I.P) for 5 days per week,the saline treated group (10 rats):UUO+saline 100μg/(kg·d) by I.P for 5 days per week,and control group (6 rats).All rats were put to death at three weeks and the renal samples were used in morphological research.Results The numbers of inflammatory cells infiltration in colchicine treated group rats significantly decreased compared to that in saline treated group (1*#738 44 ±910 87 cells/mm 2 vs. 6*#322 05 ± 1*#198 18 cells/mm 2, P< 0 001).The index of interstitial fibrosis in colchicine treated rats also significantly reduced compared to that in saline treated rats (22 67±17 61 vs.72 92±9 86, P< 0 001).Meanwhile,the tubular lesions in saline treated rats were severer than that in colchicine treated rats (2 47±0 47 vs.1 43±0 65 P< 0 05).Conclusion Colchicine had beneficial effects on interstitial fibrosis in rats with UUO Sprague Dawley.
出处
《江苏医药》
CAS
CSCD
北大核心
2002年第8期583-585,共3页
Jiangsu Medical Journal
基金
江苏省卫生厅重点资助项目 (编号 :H990 6)
江苏省教委资助项目 (编号 :99KDJ3 2 0 0 11)
