摘要
研究了固定化啤酒酵母细胞催化三甲基硅乙酮不对称还原反应 ,系统探讨了振荡速度、底物浓度、固定化细胞浓度、pH值和反应温度对反应速度、产率和产物光学纯度的影响。结果表明 ,上述因素对固定化啤酒酵母细胞催化三甲基硅乙酮不对称还原反应均有较显著的影响。振荡速度以 1 5 0r min为宜 ,底物浓度和固定化细胞浓度分别为 1 4mmol L和 0 1 5g mL较佳 ,适宜的pH值为 7 3 ,最佳反应温度为 2 5℃～ 3 0℃。在该优化反应条件下 ,反应最大产率和产物的光学纯度分别高达 84 9%和 90 2 %ee。
Asymmetric microbial reduction of acetyltrimethylsilane with immobilized Saccharomyces cerevisiae cells was studied. The effects of shake speeds, substrate concentration, immobilized cell concentration, pH and reaction temperature on the initial reaction rate, maximum yield and optical purity of the product were systematically studied. All above mentioned parameters have important effects on the raction. The optimum shake speed, substrate concentration, immobilized cell concentration, pH and reaction temperature were found to be 150r/min, 14mmol/L, 0 15g/mL, 7 3 and 25℃～30℃ respectively for the reaction, under which the maximum yield and optical purity of the product were as high as 84 9% and 90 2%ee.
出处
《微生物学报》
CAS
CSCD
北大核心
2002年第4期484-489,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金 ( 2 0 0 76 0 1 9)
广东省自然科学基金资助项目 ( 0 0 0 4 44)~~
关键词
固定化啤酒酵母细胞
催化
有机硅酮
不对称还原
Acetyltrimethylsilane, Immobilized cells, Saccharomyces cerevisiae , Asymmetric reduction,(\|)\|1\|Trimethylsilyl\|ethanol
