摘要
用关中驴制备抗犬细小病毒 (CPV)免疫球蛋白 ,预防和治疗犬CPV感染。制备CPV抗原复合物 ,通过免疫驴 ,用纯化方法从驴血清中提取特异性抗体 (IgG) ,经过物理化学检定、血清学、免疫金电镜技术、动物效力实验。免疫驴均可产生特异IgG ,免疫金电镜可观察到抗原抗体免疫复合物 ;血凝抑制试验 (HI)≥1∶512(IgG浓缩浓度为60mg/mL) ;可保护犬免受CPV攻击 ;室温保存半年 ,4℃两年效价不变。已成功地应用CPV免疫驴制备出高效价特异性抗CPV -IgG。
Anti-canine parvovirus(CPV)IgG was prepaned by intra-muscular or subcutaneous injection of donkeys with CPV compound antigen.Donkey sera were collected 12 days after booster immunization and extracted for CPV-specific IgG,as identified by physical,chamical detection,serology,immunogold electron-microscopy and animal experiments.The purifid and concentrated IgG produsts remained stable for 6 months at room temperature and for 2 years at 4℃.The CPV-IgG could protect suckling dogs from CPV challenge.It was shown that the immunized donkeys could produce large amount of CPV specific IgG with high titer(≥1:512)
出处
《中国动物检疫》
CAS
2002年第8期23-24,共2页
China Animal Health Inspection
