摘要
本文报道了不同理化因子对新疆紫草(Arnebia euchroma)愈伤组织生长及紫草宁衍生物生物合成的影响。试验结果表明,有利于愈伤组织生长和色素形成的合适温度为25℃;培养基pH值的最适范围为5.3—5.8;白光对愈伤组织的生长和紫草宁衍生物的形成具有强烈的抑制作用;培养基中添加0.2mg/L的IAA和0.5mg/L的KT对愈伤组织的生长具有促进作用;IAA和KT对紫草宁衍生物合成无促进作用,并随两者浓度的提高,培养物中紫草宁衍生物的含量与产量逐渐降低。
The effects of some physical and chemical factors on callus growth and shikonin derivative formation in the callus cultures of Arnebia euchroma were discussed.According to experiments, the optimum temperature for callus growth and shikonin derivative formation was 25℃,and the favorable initial pH of media was in the range of 5.3—5.8.Authors also found that both callus growth and shikonin derivative formation were strongly inhibited by white light. Callus growth was promoted when 0.2 mg/L IAA and 0.5 mg/L KT were added to the media, but IAA and KT did not promote shikonin derivative formation.Furthermore,the content and. yield of shikonin derivatives in cultures decreased in company with the increase of IAA and KT concentration in the media.
基金
国家“七五”攻关课题
关键词
紫草
愈伤组织
紫草宁衍生物
培养
Arnebia euchroma
Callus culture
shikonin derivatives
Physical and chemical factors
