摘要
小麦叶绿体膜蛋白经NaCl解离后,0-30%饱和度的硫酸铵沉淀组分过BA-sepharose6B柱,6BA溶液专一冼脱结合部分,结合蛋白经聚丙烯酰胺凝胶电泳,银染色呈单带,分子量约为250kD,SDS-聚丙烯酰胺凝胶电泳呈现两条带,分子量分别为66kD和60kD。结果表明,小麦叶绿体中的CTK结合蛋白可能是由两种不同亚基组成的。
The extract of wheat chloroplast membrane proteins was precipitated by different saturation of (NH_4)_2SO_4. Pellet of 0-30% saturation showing high binding activity to ^(?)H-6BA was loaded on the affinity chromatography column which was prepared by coupling 6BA to epoxy activated sepharose 6B. The CTK-binding protein was eluted from the BA-sepharose 6B column with Tris buffer containing 0.1 mmol/L 6BA. It showed a single protein band on PAGE and the apparent molecular weight was about 250kD. Two bands with molecular weight of 60kD and 66kD were detected on SDS-PAGE. It was supposed that the protomer of CTK-binding protein was a tetramer of two subunits.
基金
国家自然科学基金资助项目
关键词
小麦
叶绿体
CTK结合蛋白
提纯
Wheat chloroplast
Cytokinin-binding protein
Affinity chromatography
