摘要
受一定浓度SO_2熏气的大豆幼苗出现可见伤害以后,在继续熏气的过程中可见伤害程度不再进一步发展,表现出一定的适应性。与此相联系,膜透性增加和TTC还原力下降这两个SO_2伤害指标也得到一定程度的恢复,SO_2熏气使游离氨基酸含量增加,随着熏气时间延长,增加的游离氨基酸含量回到对照水平,提示受扰乱的代谢过程有所恢复。低浓度SO_2预处理提高了大豆对高浓度SO_2的抗性,与抗氧化有关的巯基(-SH)含量显著增加,超氧物歧化酶(SOD)活性也有所增强,同工酶谱分析显示有SOD同工酶带的酶量增加或新带出现。
After visible injury appeared in the soybean seedlings fumigated with certain concentrations of SO_2, the extent of injury ceased to increase as the fumigation continued, so the seedlings showed a certain degree of adaptation to SO_2. In relation to this phenomenon, two indexes of SO_2 injury, the increase of membrane permeability and the decline of TTCreduction ability, also showed some degree of recovery. SO_2 fumigation induced an increase of free amino acid content, which decreased to the level of the control with the prolongation of fumigation. It is suggested that the perturbed metabolic processes recovered somewhat. Pretreating with low SO_2 concentration increased the resistance of soybean seedlings to high SO_2 concentration. The content of SH group in leaves increased evidently and the SOD activity also increased to some extent. The analysis of isozymes showed that the level of two bands of SOD isozyme increased, and they sometimes didn't appear in the control. These increases showed an intensifying increase of the antioxidative ability which implied an increase of plant resistance to SO_2.
基金
国家自然科学基金
关键词
大豆
二氧化硫
适应性反应
SO_2
soybean
adaptive response
membrane permeability
TTC reductive ability
SH-group
SOD
