摘要
伸展蛋白(Extensin)作为一种细胞壁蛋白被认为是与植物的抗病性有关。利用胡萝卜愈伤组织伸展蛋白的抗体,借助于免疫学交叉反应,对大豆下胚轴中伸展蛋白的种类及诱导因素差异引起不同伸展蛋白的合成进行了研究。Western Blotting分析结果表明,0.2MCaCl2可以从大豆下胚轴细胞壁中提取得到一种细胞壁蛋白质组分,这个组分不仅在电泳性质及免疫原性质上与胡萝卜愈伤组织伸展蛋白相同,并且在与细胞壁结合的性质上也相同。真菌诱导物处理的大豆下胚轴中总的伸展蛋白的积累高于受伤处理的大豆下胚轴,并且差异主要是由于与胡萝卜伸展蛋白相同电泳性质蛋白质组分造成的。利用Western Blotting分析和斑点-酶联免疫吸附分析观察不同抗灰斑病品系的大豆下胚轴积累伸展蛋白的速度发现,抗性品系(合丰29和丰收10)的大豆下胚轴可以较迅速积累伸展蛋白(在约24—48小时),而感病品系大豆下胚轴(绥农4)则积累伸展蛋白较晚(约在72小时)。这些结果表明细胞壁伸展蛋白的积累快慢可能是造成大豆对灰斑病表现抗性还是感病的因素之一。
Extensin, as a kind of primary cell wall protein, is considered to be related to disease resistance of plant. By using antibody against extensin from carrot callus, the type of extensin in hypocotyl of soybean and their syntheses induced by fungal elicitor have been studied. Western blotting analysis shows that a CaCl_2-extracted protein from cell wall of soybean hypocotyl is electrophoreticalty and immunologically similar to the extensin in carrot callus. And the composition of cell wall binding extensin has the similar character to that in carrot callus. The accumulation of total extensin from soybean hypocotyl treated with the fungal elicitor shows a higher level than that treated with mechanical injury.By means of Western Blotting and Dot-ELISA analyses, the accumulation of extensin in various soybean strains which are resistant ro sensitive to Cercospora sojina Hara has been studied under the treatment with the fungal elicitor. It shows that the resistance to Cercospora sojina in soybean hypocotyl may be resulted from the extensin sythesis.
出处
《植物病理学报》
CAS
CSCD
北大核心
1991年第3期217-222,共6页
Acta Phytopathologica Sinica
