摘要
将无花果曲霉 (A .ficuum)AS3 .3 2 4的植酸酶基因 (phyA) ,克隆到pPIC9K中 ,得到重组载体pPIC9K/phyAⅡ ,分别用限制性内切酶DraⅠ和Bpu1 1 0 2Ⅰ线性化 ,用电穿孔转化方法导入宿主巴斯德毕赤酵母 (Pichiapastoris)GS1 1 5 中 ,构建了两种植酸酶工程酵母。本文比较了这两种工程菌表达的植酸酶的酶学性质。研究结果表明 。
The gene phyA of A.ficuum AS3.324 is cloned to pPIC9K. The ecombinant vector pPIC9K/phyAⅡ is linearized with DraⅠ or Bpu1102Ⅰ,and transformed into Pichia pastoris GS115 by electroporation. This article reports the study about the properties of phytases expressed by two different engineering yeasts. It shows that their enzyme properties are similar.
出处
《大连轻工业学院学报》
2002年第2期128-131,共4页
Journal of Dalian Institute of Light Industry
