摘要
目的 :研究细胞色素P4 5 0ⅡE1 (CYPⅡE1 )基因多态性与药物性肝损害发生发展的关系。方法 :从 4 3例药物性肝病患者及 5 0例正常人 (对照组 )外周血单个核细胞抽提基因组DNA ,采用PCR RFLP方法对CYPⅡE1基因分型。结果 :CYPⅡE1可分为A型 (c1纯合子 )、B型 (c1 /c2杂合子 )和C型 (c2纯合子 )。 5 0例正常人中c1基因频率 93% ,c2基因频率 7% ;4 3例药物性肝损害患者中c1基因频率 5 2 .3% ,c2基因频率 4 7.7% ,两组间差异显著 (P <0 .0 1 )。结论 :药物毒性反应的个体差异可能与CYPⅡE1等位基因的突变有关。
Objective:To study the relationship between genotypes of cytochrome P450ⅡE1 (CYPⅡE1) and development of drug induced liver injury.Methods:After extraction of DNA from peripheral blood mononuclear cells(PBMC),genotypes of CYPⅡE1 were determined by PCR RFLP.Results:The genotypes were separated into three types:type A (homozygous for c1 gene),type B(heterozygous for c1/c2 gene)and type C(homozygous for c2 gene).In 50 healthy control, c1 frequency was 93% and c2 frequency was 7%.In 43 patients with drug induced liver injury group, c1 was 52.3% and c2 was 47.7%. Compared drug induced liver injury group with healthy control, c1 decreased and c2 increased significantly( P <0.01).Conclusion:c2 gene might be play a role in drug induced liver injury.
出处
《中日友好医院学报》
2002年第2期67-69,F002,共4页
Journal of China-Japan Friendship Hospital
