摘要
用编码抑制素 α(1～ 32 )的基因与 pc DNA3.1真核表达质粒进行重组 ,构建抑制素基因疫苗 p INH。将 30只 2周龄 ICR小鼠随机分为 3组 (每组 10只 ) ,试验 、 组分别经脂质体介导肌肉注射 p INH 15μg(10 0μL )、2 5μg(10 0μL) ,对照组注射免疫空载体 pc DNA3.115 μg(10 0 μL)。首次免疫后间隔 3周加强免疫 1次。小鼠经 p INH基因免疫后 ,用 EL ISA可从血浆中检测到抑制素抗体 ,表明 p INH可在活体肌细胞内表达 ,表达产物具有抑制素抗原性 ,能激活免疫系统产生抑制素抗体。抑制素抗体 P/ N>2视为阳性 ,阳性率为 30 % (6 / 2 0 )。抑制素抗体阳性小鼠与阴性小鼠的产仔数、初生重和窝重等没有显著差异 (P>0 .0 5 )。抗体阳性组首次免疫后 ,雌鼠血浆 FSH浓度略有升高 ,经过加强免疫后显著升高 (P<0 .0 5 )。由此可以看出 ,抑制素基因免疫可以诱导产生抗抑制素抗体 。
Gene encoding inhibin α(1~32) was recombined with pcDNA3 1 expression vector to construct inhibin gene vaccine pINH.Plasmid pINH mixed with liposome was injected to mouse muscle at 0 15 g/L in group Ⅰ and 0 25 g/L in group Ⅱ.The boost was conducted after 3 weeks of the primary immunization.The plasma samples were prepared before the primary immunization,boost and 3 week after the primary immunization and stored at -20℃.Inhibin antibody was detected in the plasma samples by ELISA,indicating that the inhibin gene was expressed in the muscle cells and the expressed material had inhibin antigenecity.Thirty two per cent of mice(6/20) were detected to have positive inhibin antibody,with P/N values>2.There were little differences of the litter size,birth weight and litter weight between the positive and the control group( P >0 05).Plasma FSH concentration in the positive mouse was higher than that of control group averagely after the primary immunization( P <0 05).This is the first experiment using inhibin gene immunization to control animal reproduction and the new approach is a very promising strategy.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2002年第4期368-370,共3页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目 (3 0 0 70 5 5 5 )
高校博士点基金资助项目
