摘要
以昆明小鼠为试验对象 ,研究了氯化锶 (Sr Cl2 )浓度、激活处理时间以及卵龄对卵母细胞 Sr Cl2 孤雌活化的影响。h CG注射后 18h的小鼠卵母细胞在 1.6、5 .0、10、2 0 m mol/L Sr Cl2 的激活液中处理 10 min,各组间的激活率 (原核期卵母细胞的比例 )无显著性差异 (P >0 .0 5 )。 10、2 0 mmol/L Sr Cl2 组发育至 2 -、4 -细胞期比率 (5 9.2 6 %、2 0 .37% ,6 3.79%、2 0 .6 9% )显著高于 1.6、5 .0 mm ol/L 组 (2 9.6 3%、9.2 6 % ,4 7.0 6 %、7.84 % ) (P<0 .0 5 )。只有 2 0 mmol/L 组的少数激活卵母细胞 (3.4 5 % )发育至桑椹胚阶段。 10、2 0 mmol/L Sr Cl2 组处理 2 0、4 0、6 0 min,2组间的活化率无显著性差异 ;处理 4 0 min,2 0 m mol/L 组的桑椹胚和囊胚发育率 (6 4 .4 4 % ,2 2 .2 2 % )均显著高于 10 mmol/L 组 (30 .36 % ,11.6 1% ) ;处理 6 0 min,10 mmol/L 组的桑椹胚和囊胚发育率 (84 .6 8% ,4 6 .77% )均显著高于 2 0 mmol/L 组(6 7.4 6 % ,2 8.5 7% )。 10 mmol/L Sr Cl2 处理 2 0、4 0、6 0、180、36 0 m in,其活化率和 2 -细胞的发育率无显著性差异。 6 0、180 min处理组的囊胚发育率 (49.5 8%、4 7.5 9% )差异不显著 ,但 2者均显著高于 2 0、4 0、36 0 m in组 (4.5 0 %、12 .2 4
In this study the effects of concentration of SrCl 2,duration time of exposure to SrCl 2 and oocyte age on the parthenogenetic activation of mouse oocytes were studied.In the experiment 1,the concentration of SrCl 2 on the parthenogenetic activation of mouse oocytes were studied.Mouse(Kunming) oocytes collected at post hCG injection 18 h were treated in 1 6,5 0,10 and 20 mmol/L SrCl 2 CZB for 10 min respectively,the activation rates(pronuclei rates) were not different significantly between every two groups.The 2 cell and 4 cell rates(59 26%,20 37%;63 79%,20 69%) in 10 and 20 mmol/L groups were higher significantly than those(29 63%,9 26%;47 06%,7 84%) in 1 6 and 5 0 mmol/L groups.Only minority(3 45%) in 20 mmol/L group reached morula stage.The activation rates of oocytes treated with 10 or 20 mmol/L SrCl 2 for 20 min were not different significantly,so did for 40 and 60 min treated groups.The morula and blastocyst rates(64 44%,22 22%) in the group that oocytes were treated with 20 mmol/L SrCl 2 for 40 min were higher significantly than those(30 36%,11 61%) with 10 mmol/L;but when the duration time prolong to 60 min,the morula and blastocyst rates(84 68%,46 77%) in the 10 mmol/L group were higher significantly than those(67 46%,28 57%) of 20 mmol/L group.In experiment 2,the effects of duration time of exposure to SrCl 2 on the parthenogenetic activation of mouse oocytes were studied.The activation rates and 2 cell rates were not different significantly for the oocytes treated with 10 mmol/L SrCl 2 for 20,40,60,180 and 360 min;the blastocyst rates(49 58% vs 47 95%) in 60 min group and 180 min group(treated in 10 mmol/L SrCl 2) were not different significantly,both were higher significantly than those(4 5%,12 24% and 35 53%) in 20,40 and 360 min group,the three latter differed significantly between every two groups.In experiment 3,the effect of oocyte age on oocyte parthenogenetic activation was studied.The activation rate(77 78%) of oocytes collected 16 h post hCG injection (treated with 10 mmol/L SrCl 2 for 60 min) was higher significantly than that(29 63%) of oocytes collected 14 h post hCG injection,and the activation rate(94 19%) of oocytes collected 18 h post hCG injection was higher significantly than that of oocytes collected at hCG 16 h. The blastocyst rates(6 71%,27 78%,47 67%) of oocytes collected at hCG 14,16,18 were different significantly between every two groups,the blastocyst rate(39 74%) of hCG 20 h was lower significantly than that of hCG 18 h.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2002年第4期321-324,共4页
Chinese Journal of Veterinary Science
基金
国家"九五"科技攻关资助项目 (96-A2 3 -0 6-0 5 )
